Zinc Ion Doped Solid-Phase Extraction of Eicosapentaenoic Acid and Docosahexaenoic Acid from Antarctic Krill

Baokun Tang; Minglei Tian; Kyung Ho Row

doi:10.1080/00032719.2012.702179

Zinc Ion Doped Solid-Phase Extraction of Eicosapentaenoic Acid and Docosahexaenoic Acid from Antarctic Krill

Baokun Tang, Minglei Tian, Kyung Ho Row

Inha University

Research output: Contribution to journal › Article › peer-review

Abstract

Antarctic krill crude extracts contain high levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Accordingly, the solid phase extraction of EPA and DHA from Antarctic krill crude extracts has attracted significant research interest. This study compared the extraction of EPA and DHA from Antarctic krill crude extracts using an aminopropyl, zinc ion-doped silica, and C₁₈ and zinc ion-doped C₁₈ solid-phase column. The best extraction effect was obtained using the zinc ion-doped C₁₈ SPE with water containing methanol as the eluant. The efficiency increased gradually with increasing methanol concentration from 12.5 to 25% in the washing stage, and when pure methanol (5.0 mL) or acetonitrile (3.0 mL) was used as the eluant. To detect EPA and DHA, the acids were first converted to their methyl esters and detected by gas chromatography with flame ionization detection (GC-FID). In the zinc ion-doped C₁₈ elution fractions, EPA and DHA were isolated from the crude extracts in high yield (85-91% (r² = 4.8-6.3%)).

Original language	English
Pages (from-to)	2675-2686
Number of pages	12
Journal	Analytical Letters
Volume	45
Issue number	18
DOIs	https://doi.org/10.1080/00032719.2012.702179
State	Published - Nov 2012

Bibliographical note

Funding Information:
Received 27 April 2012; accepted 4 June 2012. This study was a part of the project titled ‘‘Korea Sea Grant Program (Gyeong-gi Sea Grant)’’ funded by the Ministry of Land, Transport, and Maritime Affairs, Korea. Address correspondence to Kyung Ho Row, Department of Chemical Engineering, Inha University, Incheon 402-751, Korea. E-mail: [email protected]

Keywords

Aminopropyl
Antarctic Krill
Docosahexaenoic acid
Eicosapentaenoic acid
Zinc ion doped C
Zinc ion doped silica

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10.1080/00032719.2012.702179

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title = "Zinc Ion Doped Solid-Phase Extraction of Eicosapentaenoic Acid and Docosahexaenoic Acid from Antarctic Krill",

abstract = "Antarctic krill crude extracts contain high levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Accordingly, the solid phase extraction of EPA and DHA from Antarctic krill crude extracts has attracted significant research interest. This study compared the extraction of EPA and DHA from Antarctic krill crude extracts using an aminopropyl, zinc ion-doped silica, and C18 and zinc ion-doped C18 solid-phase column. The best extraction effect was obtained using the zinc ion-doped C18 SPE with water containing methanol as the eluant. The efficiency increased gradually with increasing methanol concentration from 12.5 to 25% in the washing stage, and when pure methanol (5.0 mL) or acetonitrile (3.0 mL) was used as the eluant. To detect EPA and DHA, the acids were first converted to their methyl esters and detected by gas chromatography with flame ionization detection (GC-FID). In the zinc ion-doped C18 elution fractions, EPA and DHA were isolated from the crude extracts in high yield (85-91% (r2 = 4.8-6.3%)).",

keywords = "Aminopropyl, Antarctic Krill, Docosahexaenoic acid, Eicosapentaenoic acid, Zinc ion doped C, Zinc ion doped silica",

author = "Baokun Tang and Minglei Tian and Row, {Kyung Ho}",

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AU - Tian, Minglei

AU - Row, Kyung Ho

N1 - Funding Information: Received 27 April 2012; accepted 4 June 2012. This study was a part of the project titled ‘‘Korea Sea Grant Program (Gyeong-gi Sea Grant)’’ funded by the Ministry of Land, Transport, and Maritime Affairs, Korea. Address correspondence to Kyung Ho Row, Department of Chemical Engineering, Inha University, Incheon 402-751, Korea. E-mail: [email protected]

PY - 2012/11

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N2 - Antarctic krill crude extracts contain high levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Accordingly, the solid phase extraction of EPA and DHA from Antarctic krill crude extracts has attracted significant research interest. This study compared the extraction of EPA and DHA from Antarctic krill crude extracts using an aminopropyl, zinc ion-doped silica, and C18 and zinc ion-doped C18 solid-phase column. The best extraction effect was obtained using the zinc ion-doped C18 SPE with water containing methanol as the eluant. The efficiency increased gradually with increasing methanol concentration from 12.5 to 25% in the washing stage, and when pure methanol (5.0 mL) or acetonitrile (3.0 mL) was used as the eluant. To detect EPA and DHA, the acids were first converted to their methyl esters and detected by gas chromatography with flame ionization detection (GC-FID). In the zinc ion-doped C18 elution fractions, EPA and DHA were isolated from the crude extracts in high yield (85-91% (r2 = 4.8-6.3%)).

AB - Antarctic krill crude extracts contain high levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Accordingly, the solid phase extraction of EPA and DHA from Antarctic krill crude extracts has attracted significant research interest. This study compared the extraction of EPA and DHA from Antarctic krill crude extracts using an aminopropyl, zinc ion-doped silica, and C18 and zinc ion-doped C18 solid-phase column. The best extraction effect was obtained using the zinc ion-doped C18 SPE with water containing methanol as the eluant. The efficiency increased gradually with increasing methanol concentration from 12.5 to 25% in the washing stage, and when pure methanol (5.0 mL) or acetonitrile (3.0 mL) was used as the eluant. To detect EPA and DHA, the acids were first converted to their methyl esters and detected by gas chromatography with flame ionization detection (GC-FID). In the zinc ion-doped C18 elution fractions, EPA and DHA were isolated from the crude extracts in high yield (85-91% (r2 = 4.8-6.3%)).

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KW - Antarctic Krill

KW - Docosahexaenoic acid

KW - Eicosapentaenoic acid

KW - Zinc ion doped C

KW - Zinc ion doped silica

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Zinc Ion Doped Solid-Phase Extraction of Eicosapentaenoic Acid and Docosahexaenoic Acid from Antarctic Krill

Abstract

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Keywords

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