Wound healing potential of antibacterial microneedles loaded with green tea extracts

So Young Park; Hyun Uk Lee; Young Chul Lee; Gun Hwa Kim; Edmond Changkyun Park; Seung Hyun Han; Jeong Gyu Lee; Saehae Choi; Nam Su Heo; Dong Lak Kim; Yun Suk Huh; Jouhahn Lee

doi:10.1016/j.msec.2014.06.021

Wound healing potential of antibacterial microneedles loaded with green tea extracts

So Young Park, Hyun Uk Lee, Young Chul Lee, Gun Hwa Kim, Edmond Changkyun Park, Seung Hyun Han, Jeong Gyu Lee, Saehae Choi, Nam Su Heo, Dong Lak Kim, Yun Suk Huh, Jouhahn Lee

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

This study evaluates the utility of an antibacterial microneedle composed of green tea (GT) extract and hyaluronic acid (HA), for the efficient delivery of GT. These microneedles have the potential to be a patient-friendly method for the conventional sustained release of drugs. In this study, a fabrication method using a mold-based technique to produce GT/HA microneedles with a maximum area of ~ 50 mm² with antibacterial properties was used to manufacture transdermal drug delivery systems. Fourier transform infrared (FTIR) spectrometry was carried out to observe the potential modifications in the microneedles, when incorporated with GT. The degradation rate of GT in GT/HA microneedles was controlled simply by adjusting the HA composition. The effects of different ratios of GT in the HA microneedles were determined by measuring the release properties. In HA microneedles loaded with 70% GT (GT70), a continuous higher release rate was sustained for 72 h. The in vitro cytotoxicity assays demonstrated that GT/HA microneedles were not generally cytotoxic to Chinese hamster ovary cells (CHO-K1), human embryonic kidney cells (293T), and mouse muscle cells (C2C12), which were treated for 12 and 24 h. Antimicrobial activity of the GT/HA microneedles was demonstrated by ~ 95% growth reduction of gram negative [Escherichia coli (E. coli), Pseudomonas putida (P. putida), and Salmonella typhimurium (S. typhimurium)] and gram positive bacteria [Staphylococcus aureus (S. Aureus) and Bacillus subtilis (B. subtilis)], with GT70. Furthermore, GT/HA microneedles reduced bacterial growth of infected wound sites in the skin and improved wound healing process of skin in rat model.

Original language	English
Pages (from-to)	757-762
Number of pages	6
Journal	Materials Science and Engineering C
Volume	42
DOIs	https://doi.org/10.1016/j.msec.2014.06.021
State	Published - 1 Sep 2014

Bibliographical note

Funding Information:
This work (Grant No. PCC238) was supported by the Business for Cooperative R&D between Industry, and Research Institute funded Korea Small and Medium Business Administration in 2012–2014.

Keywords

Antibacterial activity
Green tea extract
Hyaluronic acid
Microneedles
Transdermal drug delivery
Wound healing

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10.1016/j.msec.2014.06.021

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title = "Wound healing potential of antibacterial microneedles loaded with green tea extracts",

abstract = "This study evaluates the utility of an antibacterial microneedle composed of green tea (GT) extract and hyaluronic acid (HA), for the efficient delivery of GT. These microneedles have the potential to be a patient-friendly method for the conventional sustained release of drugs. In this study, a fabrication method using a mold-based technique to produce GT/HA microneedles with a maximum area of ~ 50 mm2 with antibacterial properties was used to manufacture transdermal drug delivery systems. Fourier transform infrared (FTIR) spectrometry was carried out to observe the potential modifications in the microneedles, when incorporated with GT. The degradation rate of GT in GT/HA microneedles was controlled simply by adjusting the HA composition. The effects of different ratios of GT in the HA microneedles were determined by measuring the release properties. In HA microneedles loaded with 70% GT (GT70), a continuous higher release rate was sustained for 72 h. The in vitro cytotoxicity assays demonstrated that GT/HA microneedles were not generally cytotoxic to Chinese hamster ovary cells (CHO-K1), human embryonic kidney cells (293T), and mouse muscle cells (C2C12), which were treated for 12 and 24 h. Antimicrobial activity of the GT/HA microneedles was demonstrated by ~ 95% growth reduction of gram negative [Escherichia coli (E. coli), Pseudomonas putida (P. putida), and Salmonella typhimurium (S. typhimurium)] and gram positive bacteria [Staphylococcus aureus (S. Aureus) and Bacillus subtilis (B. subtilis)], with GT70. Furthermore, GT/HA microneedles reduced bacterial growth of infected wound sites in the skin and improved wound healing process of skin in rat model.",

keywords = "Antibacterial activity, Green tea extract, Hyaluronic acid, Microneedles, Transdermal drug delivery, Wound healing",

author = "Park, {So Young} and Lee, {Hyun Uk} and Lee, {Young Chul} and Kim, {Gun Hwa} and Park, {Edmond Changkyun} and Han, {Seung Hyun} and Lee, {Jeong Gyu} and Saehae Choi and Heo, {Nam Su} and Kim, {Dong Lak} and Huh, {Yun Suk} and Jouhahn Lee",

note = "Funding Information: This work (Grant No. PCC238) was supported by the Business for Cooperative R&D between Industry, and Research Institute funded Korea Small and Medium Business Administration in 2012–2014. ",

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doi = "10.1016/j.msec.2014.06.021",

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journal = "Materials Science and Engineering C",

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AU - Park, So Young

AU - Lee, Hyun Uk

AU - Lee, Young Chul

AU - Kim, Gun Hwa

AU - Park, Edmond Changkyun

AU - Han, Seung Hyun

AU - Lee, Jeong Gyu

AU - Choi, Saehae

AU - Heo, Nam Su

AU - Kim, Dong Lak

AU - Huh, Yun Suk

AU - Lee, Jouhahn

N1 - Funding Information: This work (Grant No. PCC238) was supported by the Business for Cooperative R&D between Industry, and Research Institute funded Korea Small and Medium Business Administration in 2012–2014.

PY - 2014/9/1

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N2 - This study evaluates the utility of an antibacterial microneedle composed of green tea (GT) extract and hyaluronic acid (HA), for the efficient delivery of GT. These microneedles have the potential to be a patient-friendly method for the conventional sustained release of drugs. In this study, a fabrication method using a mold-based technique to produce GT/HA microneedles with a maximum area of ~ 50 mm2 with antibacterial properties was used to manufacture transdermal drug delivery systems. Fourier transform infrared (FTIR) spectrometry was carried out to observe the potential modifications in the microneedles, when incorporated with GT. The degradation rate of GT in GT/HA microneedles was controlled simply by adjusting the HA composition. The effects of different ratios of GT in the HA microneedles were determined by measuring the release properties. In HA microneedles loaded with 70% GT (GT70), a continuous higher release rate was sustained for 72 h. The in vitro cytotoxicity assays demonstrated that GT/HA microneedles were not generally cytotoxic to Chinese hamster ovary cells (CHO-K1), human embryonic kidney cells (293T), and mouse muscle cells (C2C12), which were treated for 12 and 24 h. Antimicrobial activity of the GT/HA microneedles was demonstrated by ~ 95% growth reduction of gram negative [Escherichia coli (E. coli), Pseudomonas putida (P. putida), and Salmonella typhimurium (S. typhimurium)] and gram positive bacteria [Staphylococcus aureus (S. Aureus) and Bacillus subtilis (B. subtilis)], with GT70. Furthermore, GT/HA microneedles reduced bacterial growth of infected wound sites in the skin and improved wound healing process of skin in rat model.

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KW - Antibacterial activity

KW - Green tea extract

KW - Hyaluronic acid

KW - Microneedles

KW - Transdermal drug delivery

KW - Wound healing

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ER -

Wound healing potential of antibacterial microneedles loaded with green tea extracts

Abstract

Bibliographical note

Keywords

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