Abstract
An environmental isolate of Salmonella typhi was chromosomally marked with a gfp gene encoding green fluorescence protein (GFP) isolated from Aequorea victoria. The hybrid transposon mini-Tn5 gfp was transconjugated from E. coli to S. typhi, resulting in constitutive GFP production. The survival of S. typhi GFP155 introduced into groundwater and pond water microcosms was examined by GFP-based plate counts, total cell counts, and direct viable counts. A comparison between GFP-based direct viable counts and plate counts was a good method for verifying the viable, but non-culturable (VBNC), state of S. typhi. The entry into a VBNC state of S. typhi was shown in all microcosms. S. typhi survived longer in groundwater than in pond water as both a culturable and a VBNC state. Copyright (C) 1999 Federation of European Microbiological Societies.
| Original language | English |
|---|---|
| Pages (from-to) | 257-264 |
| Number of pages | 8 |
| Journal | FEMS Microbiology Letters |
| Volume | 170 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Jan 1999 |
| Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by the G-7 Projects Grant (9-2-4) from the Ministry of Environment of the Republic of Korea. We thank the National Institute of Environmental Research of the Republic of Korea for leading the projects. We would like to thank Dr. Leo Eberl (Germany) for the gifts of gfp vectors and strains.
Keywords
- Green fluorescence protein-based direct viable count method
- Groundwater
- Salmonella typhi
- Viable but non-culturable state
- gfp