Ultrasonic differentiation of normal versus malignant breast epithelial cells in monolayer cultures

Timothy E. Doyle; Jeffrey B. Goodrich; Brady J. Ambrose; Hemang Patel; Soonjo Kwon; Lee H. Pearson

doi:10.1121/1.3499699

Ultrasonic differentiation of normal versus malignant breast epithelial cells in monolayer cultures

Timothy E. Doyle, Jeffrey B. Goodrich, Brady J. Ambrose, Hemang Patel, Soonjo Kwon, Lee H. Pearson

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Normal and malignant mammary epithelial cells were studied using laboratory measurements, wavelet analysis, and numerical simulations of monolayer cell cultures to determine whether microscopic breast cancer can be detected in vitro with high-frequency ultrasound. Pulse-echo waveforms were acquired by immersing a broadband, unfocused 50-MHz transducer in the growth media of cell culture well plates and collecting the first reflection from the well bottoms. The simulations included a multilayer pulse-reflection model and a model of two-dimensional arrays of spherical cells and nuclei. The results show that normal and malignant cells produce time-domain signals and spectral features that are significantly different.

Original language	English
Pages (from-to)	EL229-EL235
Journal	Journal of the Acoustical Society of America
Volume	128
Issue number	5
DOIs	https://doi.org/10.1121/1.3499699
State	Published - Nov 2010
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by NIH Grant No. 5R21CA131798-02.

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10.1121/1.3499699

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title = "Ultrasonic differentiation of normal versus malignant breast epithelial cells in monolayer cultures",

abstract = "Normal and malignant mammary epithelial cells were studied using laboratory measurements, wavelet analysis, and numerical simulations of monolayer cell cultures to determine whether microscopic breast cancer can be detected in vitro with high-frequency ultrasound. Pulse-echo waveforms were acquired by immersing a broadband, unfocused 50-MHz transducer in the growth media of cell culture well plates and collecting the first reflection from the well bottoms. The simulations included a multilayer pulse-reflection model and a model of two-dimensional arrays of spherical cells and nuclei. The results show that normal and malignant cells produce time-domain signals and spectral features that are significantly different.",

author = "Doyle, {Timothy E.} and Goodrich, {Jeffrey B.} and Ambrose, {Brady J.} and Hemang Patel and Soonjo Kwon and Pearson, {Lee H.}",

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AU - Doyle, Timothy E.

AU - Goodrich, Jeffrey B.

AU - Ambrose, Brady J.

AU - Patel, Hemang

AU - Kwon, Soonjo

AU - Pearson, Lee H.

N1 - Funding Information: This work was supported by NIH Grant No. 5R21CA131798-02.

PY - 2010/11

Y1 - 2010/11

N2 - Normal and malignant mammary epithelial cells were studied using laboratory measurements, wavelet analysis, and numerical simulations of monolayer cell cultures to determine whether microscopic breast cancer can be detected in vitro with high-frequency ultrasound. Pulse-echo waveforms were acquired by immersing a broadband, unfocused 50-MHz transducer in the growth media of cell culture well plates and collecting the first reflection from the well bottoms. The simulations included a multilayer pulse-reflection model and a model of two-dimensional arrays of spherical cells and nuclei. The results show that normal and malignant cells produce time-domain signals and spectral features that are significantly different.

AB - Normal and malignant mammary epithelial cells were studied using laboratory measurements, wavelet analysis, and numerical simulations of monolayer cell cultures to determine whether microscopic breast cancer can be detected in vitro with high-frequency ultrasound. Pulse-echo waveforms were acquired by immersing a broadband, unfocused 50-MHz transducer in the growth media of cell culture well plates and collecting the first reflection from the well bottoms. The simulations included a multilayer pulse-reflection model and a model of two-dimensional arrays of spherical cells and nuclei. The results show that normal and malignant cells produce time-domain signals and spectral features that are significantly different.

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Ultrasonic differentiation of normal versus malignant breast epithelial cells in monolayer cultures

Abstract

Bibliographical note

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