Ultra-performance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis

Bo Yang; Amanda Weyers; Jong Youn Baik; Eric Sterner; Susan Sharfstein; Shaker A. Mousa; Fuming Zhang; Jonathan S. Dordick; Robert J. Linhardt

doi:10.1016/j.ab.2011.04.003

Ultra-performance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis

Bo Yang, Amanda Weyers, Jong Youn Baik, Eric Sterner, Susan Sharfstein, Shaker A. Mousa, Fuming Zhang, Jonathan S. Dordick, Robert J. Linhardt

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

A high-resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultra-performance liquid chromatography in a reverse-phase ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues, and biological fluids, because it provides high sensitivity without being subject to interference from proteins, peptides, and other sample impurities.

Original language	English
Pages (from-to)	59-66
Number of pages	8
Journal	Analytical Biochemistry
Volume	415
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2011.04.003
State	Published - 1 Aug 2011
Externally published	Yes

Bibliographical note

Funding Information:
The authors gratefully acknowledge support from the National Institutes of Health ( GM38060 , HL096972 , HL101721 , and GM090127 ) and the Bioengineered Heparin Consortium .

Keywords

Biological samples
Disaccharide analysis
Heparan sulfate
Heparin
Heparin lyase
Mass spectrometry
Ultra-performance liquid chromatography

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10.1016/j.ab.2011.04.003

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title = "Ultra-performance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis",

abstract = "A high-resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultra-performance liquid chromatography in a reverse-phase ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues, and biological fluids, because it provides high sensitivity without being subject to interference from proteins, peptides, and other sample impurities.",

keywords = "Biological samples, Disaccharide analysis, Heparan sulfate, Heparin, Heparin lyase, Mass spectrometry, Ultra-performance liquid chromatography",

author = "Bo Yang and Amanda Weyers and Baik, {Jong Youn} and Eric Sterner and Susan Sharfstein and Mousa, {Shaker A.} and Fuming Zhang and Dordick, {Jonathan S.} and Linhardt, {Robert J.}",

note = "Funding Information: The authors gratefully acknowledge support from the National Institutes of Health ( GM38060 , HL096972 , HL101721 , and GM090127 ) and the Bioengineered Heparin Consortium . ",

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doi = "10.1016/j.ab.2011.04.003",

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AU - Yang, Bo

AU - Weyers, Amanda

AU - Baik, Jong Youn

AU - Sterner, Eric

AU - Sharfstein, Susan

AU - Mousa, Shaker A.

AU - Zhang, Fuming

AU - Dordick, Jonathan S.

AU - Linhardt, Robert J.

N1 - Funding Information: The authors gratefully acknowledge support from the National Institutes of Health ( GM38060 , HL096972 , HL101721 , and GM090127 ) and the Bioengineered Heparin Consortium .

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N2 - A high-resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultra-performance liquid chromatography in a reverse-phase ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues, and biological fluids, because it provides high sensitivity without being subject to interference from proteins, peptides, and other sample impurities.

AB - A high-resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultra-performance liquid chromatography in a reverse-phase ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues, and biological fluids, because it provides high sensitivity without being subject to interference from proteins, peptides, and other sample impurities.

KW - Biological samples

KW - Disaccharide analysis

KW - Heparan sulfate

KW - Heparin

KW - Heparin lyase

KW - Mass spectrometry

KW - Ultra-performance liquid chromatography

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SN - 0003-2697

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Ultra-performance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis

Abstract

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Keywords

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