TY - JOUR
T1 - Synthesis of Silver Nanoparticles and Gold Nanoparticles Used as Biosensors for the Detection of Human Serum Albumin-Diagnosed Kidney Disease
AU - Thongwattana, Tiarpa
AU - Chaiyo, Ronnakorn
AU - Ponsanti, Khanittha
AU - Tangnorawich, Benchamaporn
AU - Pratumpong, Patcharee
AU - Toommee, Surachet
AU - Jenjob, Ratchapol
AU - Yang, Su Geun
AU - Parcharoen, Yardnapar
AU - Natphopsuk, Sitakan
AU - Pechyen, Chiravoot
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2024/11
Y1 - 2024/11
N2 - Background/Objectives: This study aims to develop a screen-printed carbon electrode (SPCE) modified with silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) for the detection of human serum albumin (HSA). The objectives include utilizing green synthesis methods for nanoparticle production and evaluating the electrochemical performance of the modified electrodes. Methods: AgNPs and AuNPs were synthesized using Phulae pineapple peel extract (PPA) as a reducing agent. The nanoparticles were characterized using UV-visible spectrophotometry (UV-vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and transmission electron microscopy (TEM). The electrochemical performance of AgNP/SPCE and AuNP/SPCE was assessed by cyclic voltammetry (CV) studies, and the electrodes were functionalized with anti-HSA antibodies for HSA detection. Results: Characterization revealed spherical nanoparticles ranging from 10 to 30 nm. Both AgNP/SPCE and AuNP/SPCE demonstrated improved electrochemical performance compared to bare SPCEs. The modified sensors could detect serum albumin concentrations from 10 to 400 μg/mL, with high correlation values of 0.97 and 0.99 for AgNPs and AuNPs, respectively. Conclusions: This research demonstrates the potential of using agricultural waste for green synthesis of nanoparticles and highlights the application of AgNPs and AuNPs in developing sensitive biosensing platforms for the detection of human serum albumin.
AB - Background/Objectives: This study aims to develop a screen-printed carbon electrode (SPCE) modified with silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) for the detection of human serum albumin (HSA). The objectives include utilizing green synthesis methods for nanoparticle production and evaluating the electrochemical performance of the modified electrodes. Methods: AgNPs and AuNPs were synthesized using Phulae pineapple peel extract (PPA) as a reducing agent. The nanoparticles were characterized using UV-visible spectrophotometry (UV-vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and transmission electron microscopy (TEM). The electrochemical performance of AgNP/SPCE and AuNP/SPCE was assessed by cyclic voltammetry (CV) studies, and the electrodes were functionalized with anti-HSA antibodies for HSA detection. Results: Characterization revealed spherical nanoparticles ranging from 10 to 30 nm. Both AgNP/SPCE and AuNP/SPCE demonstrated improved electrochemical performance compared to bare SPCEs. The modified sensors could detect serum albumin concentrations from 10 to 400 μg/mL, with high correlation values of 0.97 and 0.99 for AgNPs and AuNPs, respectively. Conclusions: This research demonstrates the potential of using agricultural waste for green synthesis of nanoparticles and highlights the application of AgNPs and AuNPs in developing sensitive biosensing platforms for the detection of human serum albumin.
KW - carbon screen-printed electrode
KW - gold nanoparticles
KW - green synthesis
KW - human serum albumin (HSA)
KW - Phulae pineapple
KW - silver nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85210433921&partnerID=8YFLogxK
U2 - 10.3390/ph17111421
DO - 10.3390/ph17111421
M3 - Article
AN - SCOPUS:85210433921
SN - 1424-8247
VL - 17
JO - Pharmaceuticals
JF - Pharmaceuticals
IS - 11
M1 - 1421
ER -
