Synthesis of bacterial celluloses in multiwalled carbon nanotube-dispersed medium

Won Il Park; Hun Sik Kim; Soon Min Kwon; Young Ho Hong; Hyoung Joon Jin

doi:10.1016/j.carbpol.2009.01.021

Synthesis of bacterial celluloses in multiwalled carbon nanotube-dispersed medium

Won Il Park, Hun Sik Kim, Soon Min Kwon, Young Ho Hong, Hyoung Joon Jin

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Carbon nanotubes are considered to be the ideal multi-functional filler, although there is some debate regarding their toxicity for bio-related applications. The bacteria, Gluconacetobacter xylinum, which produce bacterial cellulose, were cultured in a multiwalled carbon nanotube (MWCNT) dispersed Hestrin and Schramm (HS) medium by shaking incubation. The MWCNTs were functionalized with polyethylene glycol to prepare a stable MWCNT-dispersed HS medium and its stability was characterized by measuring the transmittance of a pulsed near infrared light. To investigate the toxicity of the MWCNTs to bacteria, we also introduced a green fluorescent protein gene into the bacteria and observed the fluorescence via confocal microscopy to confirm the presence of live bacteria in the MWCNT-dispersed HS medium. On the bases of the electron microscopy observations, a substantial number of MWCNTs were found to be well-dispersed and attached to the surface of the bacterial cellulose fibrils.

Original language	English
Pages (from-to)	457-463
Number of pages	7
Journal	Carbohydrate Polymers
Volume	77
Issue number	3
DOIs	https://doi.org/10.1016/j.carbpol.2009.01.021
State	Published - 11 Jul 2009

Keywords

Bacterial cellulose
Gluconacetobacter xylinum
Green fluorescent protein
Multiwalled carbon nanotubes
Toxicity

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10.1016/j.carbpol.2009.01.021

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title = "Synthesis of bacterial celluloses in multiwalled carbon nanotube-dispersed medium",

abstract = "Carbon nanotubes are considered to be the ideal multi-functional filler, although there is some debate regarding their toxicity for bio-related applications. The bacteria, Gluconacetobacter xylinum, which produce bacterial cellulose, were cultured in a multiwalled carbon nanotube (MWCNT) dispersed Hestrin and Schramm (HS) medium by shaking incubation. The MWCNTs were functionalized with polyethylene glycol to prepare a stable MWCNT-dispersed HS medium and its stability was characterized by measuring the transmittance of a pulsed near infrared light. To investigate the toxicity of the MWCNTs to bacteria, we also introduced a green fluorescent protein gene into the bacteria and observed the fluorescence via confocal microscopy to confirm the presence of live bacteria in the MWCNT-dispersed HS medium. On the bases of the electron microscopy observations, a substantial number of MWCNTs were found to be well-dispersed and attached to the surface of the bacterial cellulose fibrils.",

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AU - Park, Won Il

AU - Kim, Hun Sik

AU - Kwon, Soon Min

AU - Hong, Young Ho

AU - Jin, Hyoung Joon

PY - 2009/7/11

Y1 - 2009/7/11

N2 - Carbon nanotubes are considered to be the ideal multi-functional filler, although there is some debate regarding their toxicity for bio-related applications. The bacteria, Gluconacetobacter xylinum, which produce bacterial cellulose, were cultured in a multiwalled carbon nanotube (MWCNT) dispersed Hestrin and Schramm (HS) medium by shaking incubation. The MWCNTs were functionalized with polyethylene glycol to prepare a stable MWCNT-dispersed HS medium and its stability was characterized by measuring the transmittance of a pulsed near infrared light. To investigate the toxicity of the MWCNTs to bacteria, we also introduced a green fluorescent protein gene into the bacteria and observed the fluorescence via confocal microscopy to confirm the presence of live bacteria in the MWCNT-dispersed HS medium. On the bases of the electron microscopy observations, a substantial number of MWCNTs were found to be well-dispersed and attached to the surface of the bacterial cellulose fibrils.

AB - Carbon nanotubes are considered to be the ideal multi-functional filler, although there is some debate regarding their toxicity for bio-related applications. The bacteria, Gluconacetobacter xylinum, which produce bacterial cellulose, were cultured in a multiwalled carbon nanotube (MWCNT) dispersed Hestrin and Schramm (HS) medium by shaking incubation. The MWCNTs were functionalized with polyethylene glycol to prepare a stable MWCNT-dispersed HS medium and its stability was characterized by measuring the transmittance of a pulsed near infrared light. To investigate the toxicity of the MWCNTs to bacteria, we also introduced a green fluorescent protein gene into the bacteria and observed the fluorescence via confocal microscopy to confirm the presence of live bacteria in the MWCNT-dispersed HS medium. On the bases of the electron microscopy observations, a substantial number of MWCNTs were found to be well-dispersed and attached to the surface of the bacterial cellulose fibrils.

KW - Bacterial cellulose

KW - Gluconacetobacter xylinum

KW - Green fluorescent protein

KW - Multiwalled carbon nanotubes

KW - Toxicity

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JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

IS - 3

ER -

Synthesis of bacterial celluloses in multiwalled carbon nanotube-dispersed medium

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Keywords

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