Abstract
Three anion-exchangeable, silica-confined ionic liquids were synthesized for solid phase extraction of lactic acid from fermentation broth, followed by high-performance liquid chromatography coupled to ultraviolet detection. By comparing the adsorption isotherms of lactic acid on different silica-confined ionic liquids, interactions between the lactic acid and sorbents were investigated. The adsorbed amounts were then fitted into different adsorption isotherm equations; finally, the Langmuir equation was selected. Then the imidazolium silica with the highest adsorption capacity of lactic acid was packed into a cartridge for solid phase extraction. The loading volume of the cartridge was optimized by the Langmuir equation and geometry. After washing with distilled water and eluting with 0.25 mol L-1 of an HCl solution, the lactic acid was separated from interference with a recovery yield of 91.9%. Furthermore, this kind of anion-exchangeable material exhibited potential for industrial applications and separation of other anionic bioactive compounds.
| Original language | English |
|---|---|
| Pages (from-to) | 974-979 |
| Number of pages | 6 |
| Journal | Talanta |
| Volume | 83 |
| Issue number | 3 |
| DOIs | |
| State | Published - 15 Jan 2011 |
Bibliographical note
Funding Information:This research was supported by Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science and Technology (2010-0015731).
Keywords
- Adsorption isotherm
- HPLC
- Ion-exchange
- Lactic acid
- Silica confined ionic liquid
- Solid phase extraction