Single-walled carbon nanotubes induce cell death and transcription of TNF-α in macrophages without affecting nitric oxide production

Kyong Hoon Kim; Seung Min Yeon; Hyun Gyung Kim; Hwanbum Lee; Sun Kyung Kim; Seung Hyun Han; Kyung Jin Min; Youngjoo Byun; Eun Hee Lee; Kenneth Sung Lee; Soon Hong Yuk; Un Hwan Ha; Yong Woo Jung

doi:10.1007/s10753-013-9710-3

Single-walled carbon nanotubes induce cell death and transcription of TNF-α in macrophages without affecting nitric oxide production

Kyong Hoon Kim, Seung Min Yeon, Hyun Gyung Kim, Hwanbum Lee, Sun Kyung Kim, Seung Hyun Han, Kyung Jin Min, Youngjoo Byun, Eun Hee Lee, Kenneth Sung Lee, Soon Hong Yuk, Un Hwan Ha, Yong Woo Jung

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Single-walled carbon nanotubes (SWCNTs) are potent nanomaterials that have diverse shapes and features. The utilization of these molecules for drug delivery is being investigated; thus, it is important to determine whether they alter immune responses against pathogens. In this study, we show that macrophages treated with a mixture of lipopolysaccharide and SWCNTs produced normal levels of nitric oxide and inducible nitric oxide synthase mRNA. However, these treatments induced cell death, presumably via necrosis. In addition, treating cells with SWCNTs induced the expression of tumor necrosis factor-α mRNA, a potent pro-inflammatory cytokine. These results suggest that SWCNTs may influence immune responses, which could result in unexpected effects following their administration for the purpose of drug delivery.

Original language	English
Pages (from-to)	44-54
Number of pages	11
Journal	Inflammation
Volume	37
Issue number	1
DOIs	https://doi.org/10.1007/s10753-013-9710-3
State	Published - Feb 2014

Bibliographical note

Funding Information:
We thank Drs. Young In Park and Young Ho Jeon for their critical comments, and Mr. Hyun Suk Choi for his technical support. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2011-0012178) and by the MSIP (Ministry of Science, ICT & Future Planning, 122S-4-3-0434).

Keywords

cell death
lipopolysaccharides
macrophage
single-walled carbon nanotubes
tumor necrosis factor-α

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title = "Single-walled carbon nanotubes induce cell death and transcription of TNF-α in macrophages without affecting nitric oxide production",

abstract = "Single-walled carbon nanotubes (SWCNTs) are potent nanomaterials that have diverse shapes and features. The utilization of these molecules for drug delivery is being investigated; thus, it is important to determine whether they alter immune responses against pathogens. In this study, we show that macrophages treated with a mixture of lipopolysaccharide and SWCNTs produced normal levels of nitric oxide and inducible nitric oxide synthase mRNA. However, these treatments induced cell death, presumably via necrosis. In addition, treating cells with SWCNTs induced the expression of tumor necrosis factor-α mRNA, a potent pro-inflammatory cytokine. These results suggest that SWCNTs may influence immune responses, which could result in unexpected effects following their administration for the purpose of drug delivery.",

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author = "Kim, {Kyong Hoon} and Yeon, {Seung Min} and Kim, {Hyun Gyung} and Hwanbum Lee and Kim, {Sun Kyung} and Han, {Seung Hyun} and Min, {Kyung Jin} and Youngjoo Byun and Lee, {Eun Hee} and Lee, {Kenneth Sung} and Yuk, {Soon Hong} and Ha, {Un Hwan} and Jung, {Yong Woo}",

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AU - Yeon, Seung Min

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AU - Lee, Hwanbum

AU - Kim, Sun Kyung

AU - Han, Seung Hyun

AU - Min, Kyung Jin

AU - Byun, Youngjoo

AU - Lee, Eun Hee

AU - Lee, Kenneth Sung

AU - Yuk, Soon Hong

AU - Ha, Un Hwan

AU - Jung, Yong Woo

N1 - Funding Information: We thank Drs. Young In Park and Young Ho Jeon for their critical comments, and Mr. Hyun Suk Choi for his technical support. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2011-0012178) and by the MSIP (Ministry of Science, ICT & Future Planning, 122S-4-3-0434).

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Single-walled carbon nanotubes induce cell death and transcription of TNF-α in macrophages without affecting nitric oxide production

Abstract

Bibliographical note

Keywords

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