Abstract
There is a considerable industrial interest in both the scale-up and optimization of chromatographic operations used in the purification of fine chemicals and biomolecules. One of the major factors affecting the adsorption operation is the adsorption isotherm. Reversed-phase high-performance liquid chromatography (RP-HPLC) was used to measure the adsorption isotherm of phenol and o-cresol. From the experimental results, the retention times were decreased with increasing sample sizes, and the front of the peak was very stiff, so Langmuir adsorption isotherm was applied. Also Early-eluting component, phenol, in a mixture of the two components elutes faster than that in a pure component. Pulsed-injection method (PIM) was used to determine the two parameters of the Langmuir isotherm. The resulting parameters, a and b, were used to calculate the elution profiles of phenol and o-cresol in pure and mixed state. The agreement between the experimental data and calculated elution profiles was fairly good in a mixture as well as a pure component.
| Original language | English |
|---|---|
| Pages (from-to) | 625-628 |
| Number of pages | 4 |
| Journal | Korean Journal of Chemical Engineering |
| Volume | 17 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 2000 |
Bibliographical note
Funding Information:This work was performed in the High-~ity Sepal-ation Laboratory and the authors gratefully acknowledge the financial support of the Center of Advanced Bioseparation Technology at Inha University (lnchon, Korea).
Keywords
- Competitive Isotherm
- Phenol
- Pulsed-Input Method
- o-Cresol