Abstract
Three strong anion-exchange membranes (CIM QA, Q100 and HiTrap Q) were investigated for the separation of the major proteins, which were contained in whey, such as α-Lactallbumin, BSA and β-Lactoglobulin. Experiments were performed to determine the optimum mobile phase composition for separating the whey proteins using the standard chemicals of the proteins. The mobile phase was buffer A (20 mM piperazine-HCl pH 6.4) and buffer B (buffer A+l M NaCl) and the linear gradient elution changes of salt concentration were applied. The standard chemicals of the proteins were used to investigate the optimal mobile phase compositions with the three anion-exchange membranes. From the experimental results, it was found that HiTrap Q was the most effective in separating whey proteins.
Original language | English |
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Pages (from-to) | 538-541 |
Number of pages | 4 |
Journal | Korean Journal of Chemical Engineering |
Volume | 20 |
Issue number | 3 |
DOIs | |
State | Published - May 2003 |
Bibliographical note
Funding Information:The authors gratefully acknowledge the financial support of Center for Advanced Bioseparation Technology. This work was performed in the High-Purity Separation Laboratory of Inha University, Incheon, Korea.
Keywords
- Anion-exchange Membrane
- Buffer
- Optimal Mobile Phase Composition
- Whey Proteins