SB365, Pulsatilla saponin D suppresses the proliferation of human colon cancer cells and induces apoptosis by modulating the AKT/mTOR signalling pathway

Mi Kwon Son; Kyung Hee Jung; Sang Won Hong; Hee Seung Lee; Hong Mei Zheng; Myung Joo Choi; Ju Hyeon Seo; Jun Kyu Suh; Soon Sun Hong

doi:10.1016/j.foodchem.2012.07.096

SB365, Pulsatilla saponin D suppresses the proliferation of human colon cancer cells and induces apoptosis by modulating the AKT/mTOR signalling pathway

Mi Kwon Son, Kyung Hee Jung, Sang Won Hong, Hee Seung Lee, Hong Mei Zheng, Myung Joo Choi, Ju Hyeon Seo, Jun Kyu Suh, Soon Sun Hong

Inha University

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Pulsatilla koreana has been used as a traditional medicine for the treatment of several diseases. The purpose of this study was to determine if SB365, Pulsatilla saponin D isolated from the root of P. koreana inhibits the progression of colon cancer. We found that SB365 strongly suppressed the growth and proliferation of colon cancer cells and induced their apoptosis. Also, SB365 showed anti-angiogenic activity by decreasing the expression of HIF-1α and VEGF. These results were confirmed by an in vivo study showing that SB365 significantly inhibited tumor growth by the induction of apoptosis and inhibition of angiogenesis with stronger anticancer activity than 5-FU. When further examined for its anticancer mechanism, SB365 effectively suppressed the AKT/mTOR pathway both in vitro and in vivo. Taken together, our study demonstrated that SB365 inhibits the AKT/mTOR pathway, leading to the suppression of tumor growth and angiogenesis together with induction of apoptosis. Therefore, SB365 is a good candidate as a natural product for use in the treatment of colon cancer.

Original language	English
Pages (from-to)	26-33
Number of pages	8
Journal	Food Chemistry
Volume	136
Issue number	1
DOIs	https://doi.org/10.1016/j.foodchem.2012.07.096
State	Published - 1 Jan 2013
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by a grant from the Korea Health technology R&D Project, Ministry of Health & Welfare, Republic of Korea. (National Research Center for Sexual Medicine, A110076).

Keywords

Angiogenesis
Apoptosis
Colon cancer
Pulsatilla saponin D
SB365

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.foodchem.2012.07.096

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title = "SB365, Pulsatilla saponin D suppresses the proliferation of human colon cancer cells and induces apoptosis by modulating the AKT/mTOR signalling pathway",

abstract = "Pulsatilla koreana has been used as a traditional medicine for the treatment of several diseases. The purpose of this study was to determine if SB365, Pulsatilla saponin D isolated from the root of P. koreana inhibits the progression of colon cancer. We found that SB365 strongly suppressed the growth and proliferation of colon cancer cells and induced their apoptosis. Also, SB365 showed anti-angiogenic activity by decreasing the expression of HIF-1α and VEGF. These results were confirmed by an in vivo study showing that SB365 significantly inhibited tumor growth by the induction of apoptosis and inhibition of angiogenesis with stronger anticancer activity than 5-FU. When further examined for its anticancer mechanism, SB365 effectively suppressed the AKT/mTOR pathway both in vitro and in vivo. Taken together, our study demonstrated that SB365 inhibits the AKT/mTOR pathway, leading to the suppression of tumor growth and angiogenesis together with induction of apoptosis. Therefore, SB365 is a good candidate as a natural product for use in the treatment of colon cancer.",

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author = "Son, {Mi Kwon} and Jung, {Kyung Hee} and Hong, {Sang Won} and Lee, {Hee Seung} and Zheng, {Hong Mei} and Choi, {Myung Joo} and Seo, {Ju Hyeon} and Suh, {Jun Kyu} and Hong, {Soon Sun}",

note = "Funding Information: This study was supported by a grant from the Korea Health technology R&D Project, Ministry of Health & Welfare, Republic of Korea. (National Research Center for Sexual Medicine, A110076).",

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doi = "10.1016/j.foodchem.2012.07.096",

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AU - Son, Mi Kwon

AU - Jung, Kyung Hee

AU - Hong, Sang Won

AU - Lee, Hee Seung

AU - Zheng, Hong Mei

AU - Choi, Myung Joo

AU - Seo, Ju Hyeon

AU - Suh, Jun Kyu

AU - Hong, Soon Sun

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N2 - Pulsatilla koreana has been used as a traditional medicine for the treatment of several diseases. The purpose of this study was to determine if SB365, Pulsatilla saponin D isolated from the root of P. koreana inhibits the progression of colon cancer. We found that SB365 strongly suppressed the growth and proliferation of colon cancer cells and induced their apoptosis. Also, SB365 showed anti-angiogenic activity by decreasing the expression of HIF-1α and VEGF. These results were confirmed by an in vivo study showing that SB365 significantly inhibited tumor growth by the induction of apoptosis and inhibition of angiogenesis with stronger anticancer activity than 5-FU. When further examined for its anticancer mechanism, SB365 effectively suppressed the AKT/mTOR pathway both in vitro and in vivo. Taken together, our study demonstrated that SB365 inhibits the AKT/mTOR pathway, leading to the suppression of tumor growth and angiogenesis together with induction of apoptosis. Therefore, SB365 is a good candidate as a natural product for use in the treatment of colon cancer.

AB - Pulsatilla koreana has been used as a traditional medicine for the treatment of several diseases. The purpose of this study was to determine if SB365, Pulsatilla saponin D isolated from the root of P. koreana inhibits the progression of colon cancer. We found that SB365 strongly suppressed the growth and proliferation of colon cancer cells and induced their apoptosis. Also, SB365 showed anti-angiogenic activity by decreasing the expression of HIF-1α and VEGF. These results were confirmed by an in vivo study showing that SB365 significantly inhibited tumor growth by the induction of apoptosis and inhibition of angiogenesis with stronger anticancer activity than 5-FU. When further examined for its anticancer mechanism, SB365 effectively suppressed the AKT/mTOR pathway both in vitro and in vivo. Taken together, our study demonstrated that SB365 inhibits the AKT/mTOR pathway, leading to the suppression of tumor growth and angiogenesis together with induction of apoptosis. Therefore, SB365 is a good candidate as a natural product for use in the treatment of colon cancer.

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SB365, Pulsatilla saponin D suppresses the proliferation of human colon cancer cells and induces apoptosis by modulating the AKT/mTOR signalling pathway

Abstract

Bibliographical note

Keywords

UN SDGs

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