Ratiometric fluorescence detection of Hg(II) in aqueous solutions at physiological pH and live cells with a chemosensor based on tyrosine

Da Hye Kim; Junho Seong; Hyunsook Lee; Keun Hyeung Lee

doi:10.1016/j.snb.2014.02.029

Ratiometric fluorescence detection of Hg(II) in aqueous solutions at physiological pH and live cells with a chemosensor based on tyrosine

Da Hye Kim, Junho Seong, Hyunsook Lee, Keun Hyeung Lee

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

A fluorescent chemosensor (Pyr-Tyr) based on tyrosine showed highly selective and sensitive ratiometric response to Hg(II) among 14 metal ions in buffered aqueous solutions. The emission intensity ratio at 480 and 383 nm increased significantly from 0 to about 1.5 with the increase of Hg(II) concentrations (0-1 equiv.) and the ratiometric response to Hg(II) was not interfered by other metal ions such as Cu(II), Cd(II), and Ag(I). The detection limit of the chemosensor for Hg(II) was calculated to be 12 ± 1 nM (2.4 ppb). Pyr-Tyr exhibited ratiometric responses to Hg(II) in a wide range of pH values (pH 4.5-11.5) and the chemosensor was demonstrated to detect intracellular Hg(II) in HeLa cells. The binding mode of the chemosensor with Hg(II) was investigated by ¹H NMR titration and pH titration experiments, and ESI mass spectrometry.

Original language	English
Pages (from-to)	421-428
Number of pages	8
Journal	Sensors and Actuators B: Chemical
Volume	196
DOIs	https://doi.org/10.1016/j.snb.2014.02.029
State	Published - Jun 2014

Bibliographical note

Funding Information:
This work was supported by a grant ( 2012R1A1B3000574 ) from the Basic Research Program of the National Research Foundation and a grant ( 2012000540013 ) from the Korea Environmental Industry and Technology Institute .

Keywords

Chemosensor
Fluorescence
Hg(II)
Ratiometric
Selective
Sensor

Access to Document

10.1016/j.snb.2014.02.029

Cite this

@article{06013b8017d04c6986bf1678a9ea40f3,

title = "Ratiometric fluorescence detection of Hg(II) in aqueous solutions at physiological pH and live cells with a chemosensor based on tyrosine",

abstract = "A fluorescent chemosensor (Pyr-Tyr) based on tyrosine showed highly selective and sensitive ratiometric response to Hg(II) among 14 metal ions in buffered aqueous solutions. The emission intensity ratio at 480 and 383 nm increased significantly from 0 to about 1.5 with the increase of Hg(II) concentrations (0-1 equiv.) and the ratiometric response to Hg(II) was not interfered by other metal ions such as Cu(II), Cd(II), and Ag(I). The detection limit of the chemosensor for Hg(II) was calculated to be 12 ± 1 nM (2.4 ppb). Pyr-Tyr exhibited ratiometric responses to Hg(II) in a wide range of pH values (pH 4.5-11.5) and the chemosensor was demonstrated to detect intracellular Hg(II) in HeLa cells. The binding mode of the chemosensor with Hg(II) was investigated by 1H NMR titration and pH titration experiments, and ESI mass spectrometry.",

keywords = "Chemosensor, Fluorescence, Hg(II), Ratiometric, Selective, Sensor",

author = "Kim, {Da Hye} and Junho Seong and Hyunsook Lee and Lee, {Keun Hyeung}",

note = "Funding Information: This work was supported by a grant ( 2012R1A1B3000574 ) from the Basic Research Program of the National Research Foundation and a grant ( 2012000540013 ) from the Korea Environmental Industry and Technology Institute . ",

year = "2014",

month = jun,

doi = "10.1016/j.snb.2014.02.029",

language = "English",

volume = "196",

pages = "421--428",

journal = "Sensors and Actuators B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Ratiometric fluorescence detection of Hg(II) in aqueous solutions at physiological pH and live cells with a chemosensor based on tyrosine

AU - Kim, Da Hye

AU - Seong, Junho

AU - Lee, Hyunsook

AU - Lee, Keun Hyeung

N1 - Funding Information: This work was supported by a grant ( 2012R1A1B3000574 ) from the Basic Research Program of the National Research Foundation and a grant ( 2012000540013 ) from the Korea Environmental Industry and Technology Institute .

PY - 2014/6

Y1 - 2014/6

N2 - A fluorescent chemosensor (Pyr-Tyr) based on tyrosine showed highly selective and sensitive ratiometric response to Hg(II) among 14 metal ions in buffered aqueous solutions. The emission intensity ratio at 480 and 383 nm increased significantly from 0 to about 1.5 with the increase of Hg(II) concentrations (0-1 equiv.) and the ratiometric response to Hg(II) was not interfered by other metal ions such as Cu(II), Cd(II), and Ag(I). The detection limit of the chemosensor for Hg(II) was calculated to be 12 ± 1 nM (2.4 ppb). Pyr-Tyr exhibited ratiometric responses to Hg(II) in a wide range of pH values (pH 4.5-11.5) and the chemosensor was demonstrated to detect intracellular Hg(II) in HeLa cells. The binding mode of the chemosensor with Hg(II) was investigated by 1H NMR titration and pH titration experiments, and ESI mass spectrometry.

AB - A fluorescent chemosensor (Pyr-Tyr) based on tyrosine showed highly selective and sensitive ratiometric response to Hg(II) among 14 metal ions in buffered aqueous solutions. The emission intensity ratio at 480 and 383 nm increased significantly from 0 to about 1.5 with the increase of Hg(II) concentrations (0-1 equiv.) and the ratiometric response to Hg(II) was not interfered by other metal ions such as Cu(II), Cd(II), and Ag(I). The detection limit of the chemosensor for Hg(II) was calculated to be 12 ± 1 nM (2.4 ppb). Pyr-Tyr exhibited ratiometric responses to Hg(II) in a wide range of pH values (pH 4.5-11.5) and the chemosensor was demonstrated to detect intracellular Hg(II) in HeLa cells. The binding mode of the chemosensor with Hg(II) was investigated by 1H NMR titration and pH titration experiments, and ESI mass spectrometry.

KW - Chemosensor

KW - Fluorescence

KW - Hg(II)

KW - Ratiometric

KW - Selective

KW - Sensor

UR - http://www.scopus.com/inward/record.url?scp=84896787723&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2014.02.029

DO - 10.1016/j.snb.2014.02.029

M3 - Article

AN - SCOPUS:84896787723

SN - 0925-4005

VL - 196

SP - 421

EP - 428

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

Ratiometric fluorescence detection of Hg(II) in aqueous solutions at physiological pH and live cells with a chemosensor based on tyrosine

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this