Quantification of blood flow velocity in the human conjunctival microvessels using deep learning‐based stabilization algorithm

The quantification of blood flow velocity in the human conjunctiva is clinically essential for assessing microvascular hemodynamics. Since the conjunctival microvessel is imaged in several seconds, eye motion during image acquisition causes motion artifacts limiting the accuracy of image segmentation performance and measurement of the blood flow velocity. In this paper, we introduce a novel customized optical imaging system for human conjunctiva with deep learning‐based segmentation and motion correction. The image segmentation process is performed by the Attention‐ UNet structure to achieve high‐performance segmentation results in conjunctiva images with motion blur. Motion correction processes with two steps—registration and template matching—are used to correct for large displacements and fine movements. The image displacement values de-crease to 4–7 μm during registration (first step) and less than 1 μm during template matching (second step). With the corrected images, the blood flow velocity is calculated for selected vessels considering temporal signal variances and vessel lengths. These methods for resolving motion artifacts contribute insights into studies quantifying the hemodynamics of the conjunctiva, as well as other tissues.