Protoplast fusion between Lentinula edodes and Coriolus versicolor

Chaekyun Kim; Eung Chil Choi; Byong Kak Kim

doi:10.1007/BF02973938

Protoplast fusion between Lentinula edodes and Coriolus versicolor

Chaekyun Kim
, Eung Chil Choi
, Byong Kak Kim

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Protoplast fusion between isoleucine-, arginine- and thymidine-requiring auxotroph (Ile^-, Arg^-, Thy^-) of Lentinula edodes and arginine-requiring auxotroph (Arg^-) of Coriolus versicolor has been achieved using 30% polyethylene glycol (M.W. 4000) in 10 mM CaCl₂-glycine solution (pH 8.0). Fusion hybrids were selected in the 0.6 M sucrose supplemented minimal media on the basis of nutritional complementation with fusion frequency of 7.4×10^-6. The hybrids included both parental and non-parental types in colony morphology, growth rate and isozyme patterns. We succeeded inter-order protoplast fusion between the auxotrophs of Lentinula edodes and Coriolus versicolor overcoming the natural barriers of incompatibility. We examined the characteristics of the hybrids and clarified the fusion process using electron microscopy.

Original language	English
Pages (from-to)	448-453
Number of pages	6
Journal	Archives of Pharmacal Research
Volume	20
Issue number	5
DOIs	https://doi.org/10.1007/BF02973938
State	Published - Oct 1997
Externally published	Yes

Bibliographical note

Funding Information:
This research was supported in part by a grant from Korea Science and Engineering Foundation via the Research Center for New Drug Development, Seoul National University.

Keywords

Coriolus versicolor
Inter-order protoplast fusion
Isozyme pattern
Lentinula edodes

Access to Document

10.1007/BF02973938

Cite this

@article{b5bf75829fc14d7789c8de14ad963318,

title = "Protoplast fusion between Lentinula edodes and Coriolus versicolor",

abstract = "Protoplast fusion between isoleucine-, arginine- and thymidine-requiring auxotroph (Ile-, Arg-, Thy-) of Lentinula edodes and arginine-requiring auxotroph (Arg-) of Coriolus versicolor has been achieved using 30\% polyethylene glycol (M.W. 4000) in 10 mM CaCl2-glycine solution (pH 8.0). Fusion hybrids were selected in the 0.6 M sucrose supplemented minimal media on the basis of nutritional complementation with fusion frequency of 7.4×10-6. The hybrids included both parental and non-parental types in colony morphology, growth rate and isozyme patterns. We succeeded inter-order protoplast fusion between the auxotrophs of Lentinula edodes and Coriolus versicolor overcoming the natural barriers of incompatibility. We examined the characteristics of the hybrids and clarified the fusion process using electron microscopy.",

keywords = "Coriolus versicolor, Inter-order protoplast fusion, Isozyme pattern, Lentinula edodes",

author = "Chaekyun Kim and Choi, \{Eung Chil\} and Kim, \{Byong Kak\}",

note = "Funding Information: This research was supported in part by a grant from Korea Science and Engineering Foundation via the Research Center for New Drug Development, Seoul National University.",

year = "1997",

month = oct,

doi = "10.1007/BF02973938",

language = "English",

volume = "20",

pages = "448--453",

journal = "Archives of Pharmacal Research",

issn = "0253-6269",

publisher = "Pharmaceutical Society of Korea",

number = "5",

}

TY - JOUR

T1 - Protoplast fusion between Lentinula edodes and Coriolus versicolor

AU - Kim, Chaekyun

AU - Choi, Eung Chil

AU - Kim, Byong Kak

N1 - Funding Information: This research was supported in part by a grant from Korea Science and Engineering Foundation via the Research Center for New Drug Development, Seoul National University.

PY - 1997/10

Y1 - 1997/10

N2 - Protoplast fusion between isoleucine-, arginine- and thymidine-requiring auxotroph (Ile-, Arg-, Thy-) of Lentinula edodes and arginine-requiring auxotroph (Arg-) of Coriolus versicolor has been achieved using 30% polyethylene glycol (M.W. 4000) in 10 mM CaCl2-glycine solution (pH 8.0). Fusion hybrids were selected in the 0.6 M sucrose supplemented minimal media on the basis of nutritional complementation with fusion frequency of 7.4×10-6. The hybrids included both parental and non-parental types in colony morphology, growth rate and isozyme patterns. We succeeded inter-order protoplast fusion between the auxotrophs of Lentinula edodes and Coriolus versicolor overcoming the natural barriers of incompatibility. We examined the characteristics of the hybrids and clarified the fusion process using electron microscopy.

AB - Protoplast fusion between isoleucine-, arginine- and thymidine-requiring auxotroph (Ile-, Arg-, Thy-) of Lentinula edodes and arginine-requiring auxotroph (Arg-) of Coriolus versicolor has been achieved using 30% polyethylene glycol (M.W. 4000) in 10 mM CaCl2-glycine solution (pH 8.0). Fusion hybrids were selected in the 0.6 M sucrose supplemented minimal media on the basis of nutritional complementation with fusion frequency of 7.4×10-6. The hybrids included both parental and non-parental types in colony morphology, growth rate and isozyme patterns. We succeeded inter-order protoplast fusion between the auxotrophs of Lentinula edodes and Coriolus versicolor overcoming the natural barriers of incompatibility. We examined the characteristics of the hybrids and clarified the fusion process using electron microscopy.

KW - Coriolus versicolor

KW - Inter-order protoplast fusion

KW - Isozyme pattern

KW - Lentinula edodes

UR - https://www.scopus.com/pages/publications/0013680975

U2 - 10.1007/BF02973938

DO - 10.1007/BF02973938

M3 - Article

AN - SCOPUS:0013680975

SN - 0253-6269

VL - 20

SP - 448

EP - 453

JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

IS - 5

ER -

Protoplast fusion between Lentinula edodes and Coriolus versicolor

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this