Protein tertiary structural changes visualized by time-resolved x-ray solution scattering

Sena Ahn, Kyung Hwan Kim, Youngmin Kim, Jeongho Kim, Hyotcherl Ihee

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

We obtained a solution structural model of myoglobin (Mb) formed upon the CO photolysis of MbCO by analyzing time-resolved X-ray solution scattering data. An experiment-restrained rigid-body molecular dynamics simulation was used to find the best model whose theoretical difference scattering curve gives a satisfactory agreement with the experimental data at the time delay of 10 ns. The obtained solution model shows structural changes similar to crystallographic models for MbCO →Mb but also displays a noticeable difference in that the N-terminus and F helix show larger structural changes.

Original languageEnglish
Pages (from-to)13131-13133
Number of pages3
JournalJournal of Physical Chemistry B
Volume113
Issue number40
DOIs
StatePublished - 8 Oct 2009
Externally publishedYes

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