Protein Binding Study of Perillyl Alcohol Enantiomers by High Performance Frontal Analysis

Dexian Wang; Myong Seok Song; Gengliang Yang; Kyung Ho Row

doi:10.1081/JLC-120025049

Protein Binding Study of Perillyl Alcohol Enantiomers by High Performance Frontal Analysis

Dexian Wang, Myong Seok Song, Gengliang Yang, Kyung Ho Row

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

High-performance frontal analysis (HPFA) was used for protein binding study of perillyl alcohol (POH) enantiomers to human serum albumin (HSA). The analysis was performed on a Develosil 100-Diol-5 (10 cm × 4.6mm I.D.) column. Sodium phosphate solution (pH 7.4, ionic strength 0.17) was used as the mobile phase at a flow rate of 1 mL/min. UV wave-length was set at 205 nm. An injection volume of 600 μL was chosen to ensure the drug to be eluted as a trapezoidal peak with a plateau. Experimental data were fitted by Scatchard analysis. The binding constant (K) and binding affinity(nK) of POH enantiomers to HSA were: K = 5.20 × 10⁶ (1/mol), nK = 1.74 × 10 ⁴ (1/mol) for (S)-POH and K = 3.71 × 10⁶ (1/mol), nK = 1.51 × 10⁴ (1/mol) for (R)-POH, respectively.

Original language	English
Pages (from-to)	2861-2871
Number of pages	11
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	26
Issue number	17
DOIs	https://doi.org/10.1081/JLC-120025049
State	Published - 2003

Bibliographical note

Funding Information:
The authors gratefully appreciate the financial support by the Center for Advanced Bioseparation Technology, Inha University, Korea.

Keywords

Frontal analysis
Perillyl alcohol enantiomers
Protein binding

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10.1081/JLC-120025049

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@article{57ba946195e3406dbc5ba945079557fc,

title = "Protein Binding Study of Perillyl Alcohol Enantiomers by High Performance Frontal Analysis",

abstract = "High-performance frontal analysis (HPFA) was used for protein binding study of perillyl alcohol (POH) enantiomers to human serum albumin (HSA). The analysis was performed on a Develosil 100-Diol-5 (10 cm × 4.6mm I.D.) column. Sodium phosphate solution (pH 7.4, ionic strength 0.17) was used as the mobile phase at a flow rate of 1 mL/min. UV wave-length was set at 205 nm. An injection volume of 600 μL was chosen to ensure the drug to be eluted as a trapezoidal peak with a plateau. Experimental data were fitted by Scatchard analysis. The binding constant (K) and binding affinity(nK) of POH enantiomers to HSA were: K = 5.20 × 106 (1/mol), nK = 1.74 × 10 4 (1/mol) for (S)-POH and K = 3.71 × 106 (1/mol), nK = 1.51 × 104 (1/mol) for (R)-POH, respectively.",

keywords = "Frontal analysis, Perillyl alcohol enantiomers, Protein binding",

author = "Dexian Wang and Song, {Myong Seok} and Gengliang Yang and Row, {Kyung Ho}",

note = "Funding Information: The authors gratefully appreciate the financial support by the Center for Advanced Bioseparation Technology, Inha University, Korea.",

year = "2003",

doi = "10.1081/JLC-120025049",

language = "English",

volume = "26",

pages = "2861--2871",

journal = "Journal of Liquid Chromatography and Related Technologies",

issn = "1082-6076",

publisher = "Taylor and Francis Ltd.",

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TY - JOUR

T1 - Protein Binding Study of Perillyl Alcohol Enantiomers by High Performance Frontal Analysis

AU - Wang, Dexian

AU - Song, Myong Seok

AU - Yang, Gengliang

AU - Row, Kyung Ho

N1 - Funding Information: The authors gratefully appreciate the financial support by the Center for Advanced Bioseparation Technology, Inha University, Korea.

PY - 2003

Y1 - 2003

N2 - High-performance frontal analysis (HPFA) was used for protein binding study of perillyl alcohol (POH) enantiomers to human serum albumin (HSA). The analysis was performed on a Develosil 100-Diol-5 (10 cm × 4.6mm I.D.) column. Sodium phosphate solution (pH 7.4, ionic strength 0.17) was used as the mobile phase at a flow rate of 1 mL/min. UV wave-length was set at 205 nm. An injection volume of 600 μL was chosen to ensure the drug to be eluted as a trapezoidal peak with a plateau. Experimental data were fitted by Scatchard analysis. The binding constant (K) and binding affinity(nK) of POH enantiomers to HSA were: K = 5.20 × 106 (1/mol), nK = 1.74 × 10 4 (1/mol) for (S)-POH and K = 3.71 × 106 (1/mol), nK = 1.51 × 104 (1/mol) for (R)-POH, respectively.

AB - High-performance frontal analysis (HPFA) was used for protein binding study of perillyl alcohol (POH) enantiomers to human serum albumin (HSA). The analysis was performed on a Develosil 100-Diol-5 (10 cm × 4.6mm I.D.) column. Sodium phosphate solution (pH 7.4, ionic strength 0.17) was used as the mobile phase at a flow rate of 1 mL/min. UV wave-length was set at 205 nm. An injection volume of 600 μL was chosen to ensure the drug to be eluted as a trapezoidal peak with a plateau. Experimental data were fitted by Scatchard analysis. The binding constant (K) and binding affinity(nK) of POH enantiomers to HSA were: K = 5.20 × 106 (1/mol), nK = 1.74 × 10 4 (1/mol) for (S)-POH and K = 3.71 × 106 (1/mol), nK = 1.51 × 104 (1/mol) for (R)-POH, respectively.

KW - Frontal analysis

KW - Perillyl alcohol enantiomers

KW - Protein binding

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U2 - 10.1081/JLC-120025049

DO - 10.1081/JLC-120025049

M3 - Article

AN - SCOPUS:0242290768

SN - 1082-6076

VL - 26

SP - 2861

EP - 2871

JO - Journal of Liquid Chromatography and Related Technologies

JF - Journal of Liquid Chromatography and Related Technologies

IS - 17

ER -

Protein Binding Study of Perillyl Alcohol Enantiomers by High Performance Frontal Analysis

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Keywords

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