Preparative separation of phospholipids from soybean by NP-HPLC

Kyung Ho Row; Ju Weon Lee

doi:10.1007/BF02707061

Preparative separation of phospholipids from soybean by NP-HPLC

Kyung Ho Row, Ju Weon Lee

Inha University

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Soybean phospholipids-phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylcholine (PC)-were separated by normal-phase high performance liquid chromatography. The system was operated in a gradient mode. The experimental variables were gradient time and mobile phase composition. The experimental results showed that PE, PI and PC were resolved by three step-change gradient modes which employed ternary systems of hexane/1-propanol/water (58/40/2 and 56/40/4 by vol.%) and methanol/1-propanol/water (80/18/2, by vol.%) for the gradient times of 10, 30 and 76 min, respectively.

Original language	English
Pages (from-to)	412-415
Number of pages	4
Journal	Korean Journal of Chemical Engineering
Volume	14
Issue number	5
DOIs	https://doi.org/10.1007/BF02707061
State	Published - 1997

Bibliographical note

Funding Information:
The corresponding author gratefully acknowledges the financial support of the Korea Science and Engineering Foundation (Grant No. KOSEF 975-1100-001-2) and the donation of phospholipids from Dr. S. K. Kim in Doosan Technical Center.

Keywords

HPLC
Phospholipids
Preparative Separation
Soybean

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10.1007/BF02707061

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title = "Preparative separation of phospholipids from soybean by NP-HPLC",

abstract = "Soybean phospholipids-phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylcholine (PC)-were separated by normal-phase high performance liquid chromatography. The system was operated in a gradient mode. The experimental variables were gradient time and mobile phase composition. The experimental results showed that PE, PI and PC were resolved by three step-change gradient modes which employed ternary systems of hexane/1-propanol/water (58/40/2 and 56/40/4 by vol.%) and methanol/1-propanol/water (80/18/2, by vol.%) for the gradient times of 10, 30 and 76 min, respectively.",

keywords = "HPLC, Phospholipids, Preparative Separation, Soybean",

author = "Row, {Kyung Ho} and Lee, {Ju Weon}",

note = "Funding Information: The corresponding author gratefully acknowledges the financial support of the Korea Science and Engineering Foundation (Grant No. KOSEF 975-1100-001-2) and the donation of phospholipids from Dr. S. K. Kim in Doosan Technical Center.",

year = "1997",

doi = "10.1007/BF02707061",

language = "English",

volume = "14",

pages = "412--415",

journal = "Korean Journal of Chemical Engineering",

issn = "0256-1115",

publisher = "Springer",

number = "5",

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TY - JOUR

T1 - Preparative separation of phospholipids from soybean by NP-HPLC

AU - Row, Kyung Ho

AU - Lee, Ju Weon

N1 - Funding Information: The corresponding author gratefully acknowledges the financial support of the Korea Science and Engineering Foundation (Grant No. KOSEF 975-1100-001-2) and the donation of phospholipids from Dr. S. K. Kim in Doosan Technical Center.

PY - 1997

Y1 - 1997

N2 - Soybean phospholipids-phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylcholine (PC)-were separated by normal-phase high performance liquid chromatography. The system was operated in a gradient mode. The experimental variables were gradient time and mobile phase composition. The experimental results showed that PE, PI and PC were resolved by three step-change gradient modes which employed ternary systems of hexane/1-propanol/water (58/40/2 and 56/40/4 by vol.%) and methanol/1-propanol/water (80/18/2, by vol.%) for the gradient times of 10, 30 and 76 min, respectively.

AB - Soybean phospholipids-phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylcholine (PC)-were separated by normal-phase high performance liquid chromatography. The system was operated in a gradient mode. The experimental variables were gradient time and mobile phase composition. The experimental results showed that PE, PI and PC were resolved by three step-change gradient modes which employed ternary systems of hexane/1-propanol/water (58/40/2 and 56/40/4 by vol.%) and methanol/1-propanol/water (80/18/2, by vol.%) for the gradient times of 10, 30 and 76 min, respectively.

KW - HPLC

KW - Phospholipids

KW - Preparative Separation

KW - Soybean

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U2 - 10.1007/BF02707061

DO - 10.1007/BF02707061

M3 - Article

AN - SCOPUS:2042533241

SN - 0256-1115

VL - 14

SP - 412

EP - 415

JO - Korean Journal of Chemical Engineering

JF - Korean Journal of Chemical Engineering

IS - 5

ER -

Preparative separation of phospholipids from soybean by NP-HPLC

Abstract

Bibliographical note

Keywords

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