Predicted separation of phospholipids from soybean by chromatography on silica with changes in solvent composition

Kyung H.O. Row; J. U. Weon Lee

doi:10.1081/ss-100100156

Predicted separation of phospholipids from soybean by chromatography on silica with changes in solvent composition

Kyung H.O. Row, J. U. Weon Lee

Inha University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Normal-phase HPLC was used to separate the useful phospholipids phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylcholine (PC) from soybean lecithin. The mobile phase used in this experiment consisted of hexane, isopropanol, and methanol. The step-gradient mode was applied because the three components could not be separated by isocratic mode. To find the optimum separation conditions, the concentration profiles of effluents from a column were simulated by the retention factor and the plate theory in the step-gradient mode. The retention factor was correlated by the equation In k' = A + BF + CF² + DG + EG², where the constants A, B, C, D, and E were experimentally determined. F and G are the volume fractions of isopropanol and methanol, respectively. From the calculated results, PE was separated with hexane/isopropanol/methanol (90/5/5 vol%) in the isocratic mode, while PI and PC were resolved in the operating conditions of 15 minutes of gradient time and a second mobile phase of hexane/isopropanol/methanol (50/20/30 vol%) in the step-gradient mode. The agreement between the calculated concentration profile and the experimental data was fairly good, so the methodology developed in this work can be used to obtain useful separation conditions for stepwise elution.

Original language	English
Pages (from-to)	271-286
Number of pages	16
Journal	Separation Science and Technology
Volume	35
Issue number	2
DOIs	https://doi.org/10.1081/ss-100100156
State	Published - 2000

Bibliographical note

Funding Information:
K.H.R. gratefully acknowledges the financial support of the Korea Science and Engineering Foundation (Grant KOSEF 975-1100-001-2). This work was performed in the High-Purity Separation Laboratory of Inha University, Inchon, South Korea.

Keywords

HPLC
Phospholipids
Plate theory
Separation
Step-gradient

Access to Document

10.1081/ss-100100156

Cite this

@article{569f64da3e8f45f2bd603ccd0dbc8b49,

title = "Predicted separation of phospholipids from soybean by chromatography on silica with changes in solvent composition",

abstract = "Normal-phase HPLC was used to separate the useful phospholipids phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylcholine (PC) from soybean lecithin. The mobile phase used in this experiment consisted of hexane, isopropanol, and methanol. The step-gradient mode was applied because the three components could not be separated by isocratic mode. To find the optimum separation conditions, the concentration profiles of effluents from a column were simulated by the retention factor and the plate theory in the step-gradient mode. The retention factor was correlated by the equation In k' = A + BF + CF2 + DG + EG2, where the constants A, B, C, D, and E were experimentally determined. F and G are the volume fractions of isopropanol and methanol, respectively. From the calculated results, PE was separated with hexane/isopropanol/methanol (90/5/5 vol%) in the isocratic mode, while PI and PC were resolved in the operating conditions of 15 minutes of gradient time and a second mobile phase of hexane/isopropanol/methanol (50/20/30 vol%) in the step-gradient mode. The agreement between the calculated concentration profile and the experimental data was fairly good, so the methodology developed in this work can be used to obtain useful separation conditions for stepwise elution.",

keywords = "HPLC, Phospholipids, Plate theory, Separation, Step-gradient",

author = "Row, {Kyung H.O.} and {Weon Lee}, {J. U.}",

note = "Funding Information: K.H.R. gratefully acknowledges the financial support of the Korea Science and Engineering Foundation (Grant KOSEF 975-1100-001-2). This work was performed in the High-Purity Separation Laboratory of Inha University, Inchon, South Korea.",

year = "2000",

doi = "10.1081/ss-100100156",

language = "English",

volume = "35",

pages = "271--286",

journal = "Separation Science and Technology",

issn = "0149-6395",

publisher = "Taylor and Francis Ltd.",

number = "2",

}

TY - JOUR

T1 - Predicted separation of phospholipids from soybean by chromatography on silica with changes in solvent composition

AU - Row, Kyung H.O.

AU - Weon Lee, J. U.

N1 - Funding Information: K.H.R. gratefully acknowledges the financial support of the Korea Science and Engineering Foundation (Grant KOSEF 975-1100-001-2). This work was performed in the High-Purity Separation Laboratory of Inha University, Inchon, South Korea.

PY - 2000

Y1 - 2000

N2 - Normal-phase HPLC was used to separate the useful phospholipids phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylcholine (PC) from soybean lecithin. The mobile phase used in this experiment consisted of hexane, isopropanol, and methanol. The step-gradient mode was applied because the three components could not be separated by isocratic mode. To find the optimum separation conditions, the concentration profiles of effluents from a column were simulated by the retention factor and the plate theory in the step-gradient mode. The retention factor was correlated by the equation In k' = A + BF + CF2 + DG + EG2, where the constants A, B, C, D, and E were experimentally determined. F and G are the volume fractions of isopropanol and methanol, respectively. From the calculated results, PE was separated with hexane/isopropanol/methanol (90/5/5 vol%) in the isocratic mode, while PI and PC were resolved in the operating conditions of 15 minutes of gradient time and a second mobile phase of hexane/isopropanol/methanol (50/20/30 vol%) in the step-gradient mode. The agreement between the calculated concentration profile and the experimental data was fairly good, so the methodology developed in this work can be used to obtain useful separation conditions for stepwise elution.

AB - Normal-phase HPLC was used to separate the useful phospholipids phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylcholine (PC) from soybean lecithin. The mobile phase used in this experiment consisted of hexane, isopropanol, and methanol. The step-gradient mode was applied because the three components could not be separated by isocratic mode. To find the optimum separation conditions, the concentration profiles of effluents from a column were simulated by the retention factor and the plate theory in the step-gradient mode. The retention factor was correlated by the equation In k' = A + BF + CF2 + DG + EG2, where the constants A, B, C, D, and E were experimentally determined. F and G are the volume fractions of isopropanol and methanol, respectively. From the calculated results, PE was separated with hexane/isopropanol/methanol (90/5/5 vol%) in the isocratic mode, while PI and PC were resolved in the operating conditions of 15 minutes of gradient time and a second mobile phase of hexane/isopropanol/methanol (50/20/30 vol%) in the step-gradient mode. The agreement between the calculated concentration profile and the experimental data was fairly good, so the methodology developed in this work can be used to obtain useful separation conditions for stepwise elution.

KW - HPLC

KW - Phospholipids

KW - Plate theory

KW - Separation

KW - Step-gradient

UR - http://www.scopus.com/inward/record.url?scp=0342980320&partnerID=8YFLogxK

U2 - 10.1081/ss-100100156

DO - 10.1081/ss-100100156

M3 - Article

AN - SCOPUS:0342980320

SN - 0149-6395

VL - 35

SP - 271

EP - 286

JO - Separation Science and Technology

JF - Separation Science and Technology

IS - 2

ER -

Predicted separation of phospholipids from soybean by chromatography on silica with changes in solvent composition

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this