Abstract
Cyclobutane pyrimidine dimers and monomers of uracil and thymine were separated on a C18 reversed-phase high performance liquid chromatographic column. Rate and equilibrium constants were determined by analyzing the moments of the elution curves. Based on the parameters estimated, the comparison between the experimental and calculated values was good. The experimental results showed that optimum resolutions were obtained at 1.0 mL/min of flow rate eluant.
Original language | English |
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Pages (from-to) | 15-23 |
Number of pages | 9 |
Journal | Separation Science and Technology |
Volume | 26 |
Issue number | 1 |
DOIs | |
State | Published - 1 Jan 1991 |
Externally published | Yes |