Parameter Estimation of Cyclobutane Pyrimidine Dimers and Monomers of Uracil and Thymine in Reversed-Phase High Performance Liquid Chromatography

Kyung Ho Row, Jung Ik Raw

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Cyclobutane pyrimidine dimers and monomers of uracil and thymine were separated on a C18 reversed-phase high performance liquid chromatographic column. Rate and equilibrium constants were determined by analyzing the moments of the elution curves. Based on the parameters estimated, the comparison between the experimental and calculated values was good. The experimental results showed that optimum resolutions were obtained at 1.0 mL/min of flow rate eluant.

Original languageEnglish
Pages (from-to)15-23
Number of pages9
JournalSeparation Science and Technology
Volume26
Issue number1
DOIs
StatePublished - 1 Jan 1991
Externally publishedYes

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