Abstract
The simultaneous extraction and separation of four bioactive compounds from Gelidium amansii were carried out through liquid-liquid extraction with a liquid chromatography separation. The optimum extraction conditions were established using different extraction solvents, procedures and times. The extracts were separated and determined using an analytical C18 column with a mobile phase consisting of acetonitrile-water (containing 1.0 % acetic acid) for a gradient elution of 0-30 min from 15:85-50:50 (v/v). Finally, total amount of 0.239 mg of four bioactive compounds was obtained from 1.0 g of Gelidium amansii.
Original language | English |
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Pages (from-to) | 4480-4482 |
Number of pages | 3 |
Journal | Asian Journal of Chemistry |
Volume | 23 |
Issue number | 10 |
State | Published - 2011 |
Keywords
- Bioactive compounds
- Extraction
- Gelidium amansii
- High performance liquid chromatography