Nanoporous niobium oxide for label-free detection of DNA hybridization events

Jinsub Choi; Jae Hoon Lim; Sangchul Rho; Deokjin Jahng; Jaeyoung Lee; Kyung Ja Kim

doi:10.1016/j.talanta.2007.07.007

Nanoporous niobium oxide for label-free detection of DNA hybridization events

Jinsub Choi, Jae Hoon Lim, Sangchul Rho, Deokjin Jahng, Jaeyoung Lee, Kyung Ja Kim

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

We found that DNA probes can be immobilized on anodically prepared porous niobium oxide without a chemical modification of both the DNA probes and the substrate. By using the porous niobium oxide with a positive surface charge, DNA hybridization events are detected on the basis of the blue-shift of a maximum absorption peak in UV-vis-NIR spectroscopy. The blue-shift is ascribed to the change of surface charge upon single- or double-stranded DNA. The method does not require a label and shows high sensitivity with the detection limit of the concentration of 1 nM.

Original language	English
Pages (from-to)	1056-1059
Number of pages	4
Journal	Talanta
Volume	74
Issue number	4
DOIs	https://doi.org/10.1016/j.talanta.2007.07.007
State	Published - 15 Jan 2008
Externally published	Yes

Bibliographical note

Funding Information:
Support from MOCIE (Ministry of Commerce, Industry and Energy) of Korea and KICET is gratefully acknowledged. The authors thank Dr. J.H. Chang for assistance with the measurement of UV–vis-NIR spectroscopy and Dr. S.C. Lee for stimulating discussions.

Keywords

Anodization
Biosensor
Niobium oxide
Porous oxide
Surface charge

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10.1016/j.talanta.2007.07.007

Cite this

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title = "Nanoporous niobium oxide for label-free detection of DNA hybridization events",

abstract = "We found that DNA probes can be immobilized on anodically prepared porous niobium oxide without a chemical modification of both the DNA probes and the substrate. By using the porous niobium oxide with a positive surface charge, DNA hybridization events are detected on the basis of the blue-shift of a maximum absorption peak in UV-vis-NIR spectroscopy. The blue-shift is ascribed to the change of surface charge upon single- or double-stranded DNA. The method does not require a label and shows high sensitivity with the detection limit of the concentration of 1 nM.",

keywords = "Anodization, Biosensor, Niobium oxide, Porous oxide, Surface charge",

author = "Jinsub Choi and Lim, {Jae Hoon} and Sangchul Rho and Deokjin Jahng and Jaeyoung Lee and Kim, {Kyung Ja}",

note = "Funding Information: Support from MOCIE (Ministry of Commerce, Industry and Energy) of Korea and KICET is gratefully acknowledged. The authors thank Dr. J.H. Chang for assistance with the measurement of UV–vis-NIR spectroscopy and Dr. S.C. Lee for stimulating discussions.",

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doi = "10.1016/j.talanta.2007.07.007",

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T1 - Nanoporous niobium oxide for label-free detection of DNA hybridization events

AU - Choi, Jinsub

AU - Lim, Jae Hoon

AU - Rho, Sangchul

AU - Jahng, Deokjin

AU - Lee, Jaeyoung

AU - Kim, Kyung Ja

N1 - Funding Information: Support from MOCIE (Ministry of Commerce, Industry and Energy) of Korea and KICET is gratefully acknowledged. The authors thank Dr. J.H. Chang for assistance with the measurement of UV–vis-NIR spectroscopy and Dr. S.C. Lee for stimulating discussions.

PY - 2008/1/15

Y1 - 2008/1/15

N2 - We found that DNA probes can be immobilized on anodically prepared porous niobium oxide without a chemical modification of both the DNA probes and the substrate. By using the porous niobium oxide with a positive surface charge, DNA hybridization events are detected on the basis of the blue-shift of a maximum absorption peak in UV-vis-NIR spectroscopy. The blue-shift is ascribed to the change of surface charge upon single- or double-stranded DNA. The method does not require a label and shows high sensitivity with the detection limit of the concentration of 1 nM.

AB - We found that DNA probes can be immobilized on anodically prepared porous niobium oxide without a chemical modification of both the DNA probes and the substrate. By using the porous niobium oxide with a positive surface charge, DNA hybridization events are detected on the basis of the blue-shift of a maximum absorption peak in UV-vis-NIR spectroscopy. The blue-shift is ascribed to the change of surface charge upon single- or double-stranded DNA. The method does not require a label and shows high sensitivity with the detection limit of the concentration of 1 nM.

KW - Anodization

KW - Biosensor

KW - Niobium oxide

KW - Porous oxide

KW - Surface charge

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U2 - 10.1016/j.talanta.2007.07.007

DO - 10.1016/j.talanta.2007.07.007

M3 - Article

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AN - SCOPUS:37449014462

SN - 0039-9140

VL - 74

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EP - 1059

JO - Talanta

JF - Talanta

IS - 4

ER -

Nanoporous niobium oxide for label-free detection of DNA hybridization events

Abstract

Bibliographical note

Keywords

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