Abstract
Reversed-phase high-performance liquid chromatography was successfully developed for the simultaneous and rapid separation for the main whey proteins, α-Lactalbumin and β-Lactoglobulin. This method consisted of a linear gradient of the two mobile phases of 0.1% trifluoroacetic acid in water and 0. 1% trifluoroacetic acid in acetonitrile. The total run time for this separation was approximately 30 min, and α-Lactalbumin was eluted followed by βLactoglobulin. The injection volume was fixed at 20μl and the flow rate was 1 ml 1/min. The optimum mobile phase composition and gradient conditions to separate α-Lactalbumin and β-Lactoglobulin (A+B) were experimentally obtained at the 15μm particle with a pore size of 300 Å on the linear-gradient mode.
| Original language | English |
|---|---|
| Pages (from-to) | 705-708 |
| Number of pages | 4 |
| Journal | Korean Journal of Chemical Engineering |
| Volume | 20 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 2003 |
Keywords
- Column Liquid Chromatography
- Reversed-Phase Separation
- Whey Proteins
- α-Lactalbumin
- β-Lactoglobulin