TY - JOUR
T1 - Isolation and partial characterization of a cryptic polyene gene cluster in Pseudonocardia autotrophica
AU - Lee, Mi Yeon
AU - Ji, Seon Myeong
AU - Park, Hyun Joo
AU - Han, Kyuboem
AU - Kim, Eung Soo
PY - 2006/2
Y1 - 2006/2
N2 - The polyene antibiotics, a category that includes nystatin, pimaricin, amphotericin, and candicidin, comprise a family of very promising antifungal polyketide compounds and are typically produced by soil actinomycetes. The biosynthetic gene clusters for these polyenes have been previously investigated, revealing the presence of highly similar cytochrome P450 hydroxylase (CYP) genes. Using polyene CYP-specific PCR screening with several actinomycete genomic DNAs, Pseudonocardia autotrophica was determined to contain a unique polyene-specific CYP gene. Genomic DNA library screening using the polyene-specific CYP gene probe identified a positive cosmid clone, which contained a DNA fragment of approximately 34.5 kb. The complete sequencing of this DNA fragment revealed a total of seven complete and two incomplete open reading frames, which were found to be highly similar, but still unique, when compared to previously known polyene biosynthetic genes. These results suggest that the polyene-specific screening approach may constitute an efficient method for the isolation of potentially valuable cryptic polyene biosynthetic gene clusters from various rare actinomycetes.
AB - The polyene antibiotics, a category that includes nystatin, pimaricin, amphotericin, and candicidin, comprise a family of very promising antifungal polyketide compounds and are typically produced by soil actinomycetes. The biosynthetic gene clusters for these polyenes have been previously investigated, revealing the presence of highly similar cytochrome P450 hydroxylase (CYP) genes. Using polyene CYP-specific PCR screening with several actinomycete genomic DNAs, Pseudonocardia autotrophica was determined to contain a unique polyene-specific CYP gene. Genomic DNA library screening using the polyene-specific CYP gene probe identified a positive cosmid clone, which contained a DNA fragment of approximately 34.5 kb. The complete sequencing of this DNA fragment revealed a total of seven complete and two incomplete open reading frames, which were found to be highly similar, but still unique, when compared to previously known polyene biosynthetic genes. These results suggest that the polyene-specific screening approach may constitute an efficient method for the isolation of potentially valuable cryptic polyene biosynthetic gene clusters from various rare actinomycetes.
KW - Antifungal
KW - Cryptic gene cluster
KW - Cytochrome P450 hydroxylase
KW - Polyene
KW - Polyketide
KW - Pseudonocardia
UR - http://www.scopus.com/inward/record.url?scp=30844433078&partnerID=8YFLogxK
U2 - 10.1007/s10295-005-0018-7
DO - 10.1007/s10295-005-0018-7
M3 - Article
C2 - 16096834
AN - SCOPUS:30844433078
SN - 1367-5435
VL - 33
SP - 84
EP - 87
JO - Journal of Industrial Microbiology and Biotechnology
JF - Journal of Industrial Microbiology and Biotechnology
IS - 2
ER -