Isolation and characterization of soil Streptomyces involved in 2,4- dichlorophenol oxidation

Minjin Kang, Ja Kyoung Kang, Eung Soo Kim

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Over 50 morphologically distinctive soil Streptomyces were isolated from various locations in the Yongin area in Korea and visually screened for dye- decoloring activities on an agar plate. Two Streptomyces species (AD001 and ND002) showed strong dye-decoloring activities on the plate containing congo- red and new-fuchin dyes, respectively. Also, the liquid culture supernatants of these species showed 2,4-dicholophenol (DCP) oxidation activities only in the presence of hydrogen peroxide, a characteristic of Actinomycetes lignin- peroxidase (ALiP)-P3 isoform found in dye-degrading S. viridosporus T7A and S. badius 252. Based on their dye-decoloring capabilities and the 2,4-DCP oxidation kinetic data, it is suggested that these Streptomyces secrete not- yet-characterized extracellular enzyme(s), whose activities are very similar to the ALiP-P3 enzyme.

Original languageEnglish
Pages (from-to)877-880
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume9
Issue number6
StatePublished - Dec 1999
Externally publishedYes

Keywords

  • 2,4-DCP oxidation
  • Actinomycetes lignin-peroxidase
  • Streptomyces

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