Intravital real-time confocal laser scanning microscopy for the in situ evaluation of nanocarriers

Yu Matsumoto, Takahiro Nomoto, Kazuko Toh, Horacio Cabral, Mami Murakami, R. James Christie, Hyun Jin Kim, Tadayoshi Ogura, Kanjiro Miyata, Nobuhiro Nishiyama, Tatsuya Yamasoba, Kazunori Kataoka

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

Visualization of drug/gene nanocarriers within living tissue is essential for optimization towards clinical applications. In this regard, we have established an intravital real-time confocal laser scanning microscopy (IVRTCLSM) technique with both spatial and temporal resolution for in situ evaluation of nanocarriers. In this chapter, we describe the actual setup of the IVRTCLSM in detail and review several examples analyzing the behavior of nanocarriers. Our new imaging technique can elucidate mechanisms that have not been clarified by conventional methods that require tissue to be disturbed or manipulated ex vivo. IVRTCLSM can thereby ascertain critical barriers residing in a living body and facilitate the development of nanocarriers optimized for drug/gene delivery.

Original languageEnglish
Title of host publicationCancer Targeted Drug Delivery
Subtitle of host publicationAn Elusive Dream
PublisherSpringer New York
Pages607-620
Number of pages14
Volume9781461478768
ISBN (Electronic)9781461478768
ISBN (Print)1461478758, 9781461478751
DOIs
StatePublished - 1 Jul 2013
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2013 Springer Science+Business Media New York. All rights reserved.

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