Abstract
Visualization of drug/gene nanocarriers within living tissue is essential for optimization towards clinical applications. In this regard, we have established an intravital real-time confocal laser scanning microscopy (IVRTCLSM) technique with both spatial and temporal resolution for in situ evaluation of nanocarriers. In this chapter, we describe the actual setup of the IVRTCLSM in detail and review several examples analyzing the behavior of nanocarriers. Our new imaging technique can elucidate mechanisms that have not been clarified by conventional methods that require tissue to be disturbed or manipulated ex vivo. IVRTCLSM can thereby ascertain critical barriers residing in a living body and facilitate the development of nanocarriers optimized for drug/gene delivery.
Original language | English |
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Title of host publication | Cancer Targeted Drug Delivery |
Subtitle of host publication | An Elusive Dream |
Publisher | Springer New York |
Pages | 607-620 |
Number of pages | 14 |
Volume | 9781461478768 |
ISBN (Electronic) | 9781461478768 |
ISBN (Print) | 1461478758, 9781461478751 |
DOIs | |
State | Published - 1 Jul 2013 |
Externally published | Yes |
Bibliographical note
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