TY - JOUR
T1 - Improved SERS nanoparticles for direct detection of circulating tumor cells in the blood
AU - Wu, Xiaoxia
AU - Luo, Liqiang
AU - Yang, Sugeun
AU - Ma, Xuehua
AU - Li, Yonglong
AU - Dong, Chen
AU - Tian, Yuchen
AU - Zhang, Ling'E
AU - Shen, Zheyu
AU - Wu, Aiguo
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/5/13
Y1 - 2015/5/13
N2 - The detection of circulating tumor cells (CTCs) in the blood of cancer patients is crucial for early cancer diagnosis, cancer prognosis, evaluation of the treatment effect of chemotherapy drugs, and choice of cancer treatment options. In this study, we propose new surface-enhanced Raman scattering (SERS) nanoparticles for the direct detection of CTCs in the blood. Under the optimized experimental conditions, our SERS nanoparticles exhibit satisfying performances for the direct detection of cancer cells in the rabbit blood. A good linear relationship is obtained between the SERS intensity and the concentration of cancer cells in the range of 5-500 cells/mL (R2 = 0.9935), which demonstrates that the SERS nanoparticles can be used for the quantitative analysis of cancer cells in the blood and the limit of detection is 5 cells/mL, which is lowest compared with the reported values. The SERS nanoparticles also have an excellent specificity for the detection of cancer cells in the rabbit blood. The above results reinforce that our SERS nanoparticles can be used for the direct detection of CTCs in the blood with excellent specificity and high sensitivity.
AB - The detection of circulating tumor cells (CTCs) in the blood of cancer patients is crucial for early cancer diagnosis, cancer prognosis, evaluation of the treatment effect of chemotherapy drugs, and choice of cancer treatment options. In this study, we propose new surface-enhanced Raman scattering (SERS) nanoparticles for the direct detection of CTCs in the blood. Under the optimized experimental conditions, our SERS nanoparticles exhibit satisfying performances for the direct detection of cancer cells in the rabbit blood. A good linear relationship is obtained between the SERS intensity and the concentration of cancer cells in the range of 5-500 cells/mL (R2 = 0.9935), which demonstrates that the SERS nanoparticles can be used for the quantitative analysis of cancer cells in the blood and the limit of detection is 5 cells/mL, which is lowest compared with the reported values. The SERS nanoparticles also have an excellent specificity for the detection of cancer cells in the rabbit blood. The above results reinforce that our SERS nanoparticles can be used for the direct detection of CTCs in the blood with excellent specificity and high sensitivity.
KW - blood
KW - circulating tumor cells
KW - direct detection
KW - excellent specificity
KW - high sensitivity
KW - surface-enhanced Raman scattering
UR - http://www.scopus.com/inward/record.url?scp=84929224838&partnerID=8YFLogxK
U2 - 10.1021/acsami.5b02276
DO - 10.1021/acsami.5b02276
M3 - Article
C2 - 25875511
AN - SCOPUS:84929224838
SN - 1944-8244
VL - 7
SP - 9965
EP - 9971
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
IS - 18
ER -
