Human bone marrow stem cell responses on electrospun Bombyx mori silk fibroin

Hyoung Joon Jin; Jingsong Chen; Vassilis Karageorgiou; Gregory H. Altman; David L. Kaplan

Human bone marrow stem cell responses on electrospun Bombyx mori silk fibroin

Hyoung Joon Jin, Jingsong Chen, Vassilis Karageorgiou, Gregory H. Altman, David L. Kaplan

Tufts University

Research output: Contribution to journal › Conference article › peer-review

5 Scopus citations

Abstract

Electrospinning for the formation of nanoscale diameter fibers has been explored for high performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In this study we used electrospinning for protein-based biomaterials to fabricate scaffolds from aqueous regenerated silkworm silk, Bombyx mori,. Adhesion, spreading and proliferation of human bone marrow stem cells (hBMSCs) on electrospun silk was characterized. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the electrospun silk matrices promoted hBMSC attachment and proliferation over 10 days in culture. The responses of the hBMSCs on the electrospun silk matrices, combined with the biocompatibile properties of the silk fibroin protein matrix, suggest potential for use of this biomaterial as scaffolds for tissue engineering.

Original language	English
Pages (from-to)	129-133
Number of pages	5
Journal	Materials Research Society Symposium Proceedings
Volume	735
State	Published - 2003
Externally published	Yes
Event	Bioinspired Nanoscale Hybrid Systems - Boston, MA, United States Duration: 2 Dec 2002 → 4 Dec 2002

Cite this

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title = "Human bone marrow stem cell responses on electrospun Bombyx mori silk fibroin",

abstract = "Electrospinning for the formation of nanoscale diameter fibers has been explored for high performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In this study we used electrospinning for protein-based biomaterials to fabricate scaffolds from aqueous regenerated silkworm silk, Bombyx mori,. Adhesion, spreading and proliferation of human bone marrow stem cells (hBMSCs) on electrospun silk was characterized. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the electrospun silk matrices promoted hBMSC attachment and proliferation over 10 days in culture. The responses of the hBMSCs on the electrospun silk matrices, combined with the biocompatibile properties of the silk fibroin protein matrix, suggest potential for use of this biomaterial as scaffolds for tissue engineering.",

author = "Jin, {Hyoung Joon} and Jingsong Chen and Vassilis Karageorgiou and Altman, {Gregory H.} and Kaplan, {David L.}",

year = "2003",

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volume = "735",

pages = "129--133",

journal = "Materials Research Society Symposium Proceedings",

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note = "Bioinspired Nanoscale Hybrid Systems ; Conference date: 02-12-2002 Through 04-12-2002",

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TY - JOUR

T1 - Human bone marrow stem cell responses on electrospun Bombyx mori silk fibroin

AU - Jin, Hyoung Joon

AU - Chen, Jingsong

AU - Karageorgiou, Vassilis

AU - Altman, Gregory H.

AU - Kaplan, David L.

PY - 2003

Y1 - 2003

N2 - Electrospinning for the formation of nanoscale diameter fibers has been explored for high performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In this study we used electrospinning for protein-based biomaterials to fabricate scaffolds from aqueous regenerated silkworm silk, Bombyx mori,. Adhesion, spreading and proliferation of human bone marrow stem cells (hBMSCs) on electrospun silk was characterized. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the electrospun silk matrices promoted hBMSC attachment and proliferation over 10 days in culture. The responses of the hBMSCs on the electrospun silk matrices, combined with the biocompatibile properties of the silk fibroin protein matrix, suggest potential for use of this biomaterial as scaffolds for tissue engineering.

AB - Electrospinning for the formation of nanoscale diameter fibers has been explored for high performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In this study we used electrospinning for protein-based biomaterials to fabricate scaffolds from aqueous regenerated silkworm silk, Bombyx mori,. Adhesion, spreading and proliferation of human bone marrow stem cells (hBMSCs) on electrospun silk was characterized. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the electrospun silk matrices promoted hBMSC attachment and proliferation over 10 days in culture. The responses of the hBMSCs on the electrospun silk matrices, combined with the biocompatibile properties of the silk fibroin protein matrix, suggest potential for use of this biomaterial as scaffolds for tissue engineering.

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M3 - Conference article

AN - SCOPUS:0037700600

SN - 0272-9172

VL - 735

SP - 129

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JO - Materials Research Society Symposium Proceedings

JF - Materials Research Society Symposium Proceedings

T2 - Bioinspired Nanoscale Hybrid Systems

Y2 - 2 December 2002 through 4 December 2002

ER -

Human bone marrow stem cell responses on electrospun Bombyx mori silk fibroin

Abstract

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