TY - JOUR
T1 - Granulosicoccus marinus sp. nov., isolated from Antarctic seawater, and emended description of the genus Granulosicoccus
AU - Baek, Kiwoon
AU - Choi, Ahyoung
AU - Kang, Ilnam
AU - Im, Mihye
AU - Cho, Jang Cheon
N1 - Publisher Copyright:
© 2014 IUMS.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - A Gram-staining-negative, motile by flagella, non-pigmented, poly-β-hydroxybutyrate-producing, strictly aerobic and sphere-shaped bacterium, IMCC3490T, was isolated from a coastal seawater sample from the Antarctic Peninsula. Optimal growth of strain IMCC3490T was observed at 20 °C, pH 8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain IMCC3490T belonged to the genusGranulosicoccus in the family Granulosicoccaceae. The strain was closely related to Granulosicoccus antarcticus IMCC3135T (98.8 % 16S rRNA gene sequence similarity) and Granulosicoccus coccoides Z 271T (98.5 %). The DNA–DNA relatedness values between IMCC3490T and type strains of the two species of the genus were far lower than 70 %, which indicated strain IMCC3490T is a novel genomic species of the genus Granulosicoccus. The major fatty acids of strain IMCC3490T were summed feature 8 (C18: 1ω7cand/or C18: 1ω6c) and summed feature 3 (C16: 1ω7c and/or C16: 1ω6c). The isoprenoid quinone detected was ubiquinone-8 (Q-8) and predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 61.0 mol%. On the basis of phylogenetic analyses, DNA–DNA relatedness values and phenotypic data, it is suggested that strain IMCC3490T represents a novel species of the genus Granulosicoccus, for which the name Granulosicoccus marinus sp. nov. is proposed. The type strain is IMCC3490T (= KACC 17483T = NBRC 109704T). An emended description of the genus Granulosicoccus is also provided.
AB - A Gram-staining-negative, motile by flagella, non-pigmented, poly-β-hydroxybutyrate-producing, strictly aerobic and sphere-shaped bacterium, IMCC3490T, was isolated from a coastal seawater sample from the Antarctic Peninsula. Optimal growth of strain IMCC3490T was observed at 20 °C, pH 8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain IMCC3490T belonged to the genusGranulosicoccus in the family Granulosicoccaceae. The strain was closely related to Granulosicoccus antarcticus IMCC3135T (98.8 % 16S rRNA gene sequence similarity) and Granulosicoccus coccoides Z 271T (98.5 %). The DNA–DNA relatedness values between IMCC3490T and type strains of the two species of the genus were far lower than 70 %, which indicated strain IMCC3490T is a novel genomic species of the genus Granulosicoccus. The major fatty acids of strain IMCC3490T were summed feature 8 (C18: 1ω7cand/or C18: 1ω6c) and summed feature 3 (C16: 1ω7c and/or C16: 1ω6c). The isoprenoid quinone detected was ubiquinone-8 (Q-8) and predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 61.0 mol%. On the basis of phylogenetic analyses, DNA–DNA relatedness values and phenotypic data, it is suggested that strain IMCC3490T represents a novel species of the genus Granulosicoccus, for which the name Granulosicoccus marinus sp. nov. is proposed. The type strain is IMCC3490T (= KACC 17483T = NBRC 109704T). An emended description of the genus Granulosicoccus is also provided.
UR - http://www.scopus.com/inward/record.url?scp=84916238764&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.070045-0
DO - 10.1099/ijs.0.070045-0
M3 - Article
C2 - 25238763
AN - SCOPUS:84916238764
SN - 1466-5026
VL - 64
SP - 4103
EP - 4108
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
ER -