Freshwater Viral Metagenome Analyses Targeting dsDNA Viruses

Kira Moon; Jang Cheon Cho

doi:10.1007/978-1-0716-3515-5_3

Freshwater Viral Metagenome Analyses Targeting dsDNA Viruses

Kira Moon, Jang Cheon Cho

Honam National Institute of Biological Resources

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Viral metagenomics is one of the most widely used approaches to study viral population genomics. With the recent development of bioinformatic tools, the number of molecular biological methods, programs, and software to analyze viral metagenome data have greatly increased. Here, we describe the basic analysis workflow along with bioinformatic tools that can be used to analyze viral metagenome data. Although this chapter assumes that the viral metagenome data are prepared from the freshwater samples and are subjected to dsDNA sequencing, the protocol can be applied and modified for other types of metagenome data collected from a variety of sources.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	29-44
Number of pages	16
DOIs	https://doi.org/10.1007/978-1-0716-3515-5_3
State	Published - 2024

Publication series

Name	Methods in Molecular Biology
Volume	2732
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.

Keywords

Bacteriophage
Freshwater
Viral metagenome
Virome
dsDNA phage

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10.1007/978-1-0716-3515-5_3

Cite this

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AB - Viral metagenomics is one of the most widely used approaches to study viral population genomics. With the recent development of bioinformatic tools, the number of molecular biological methods, programs, and software to analyze viral metagenome data have greatly increased. Here, we describe the basic analysis workflow along with bioinformatic tools that can be used to analyze viral metagenome data. Although this chapter assumes that the viral metagenome data are prepared from the freshwater samples and are subjected to dsDNA sequencing, the protocol can be applied and modified for other types of metagenome data collected from a variety of sources.

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KW - Viral metagenome

KW - Virome

KW - dsDNA phage

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T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

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ER -

Freshwater Viral Metagenome Analyses Targeting dsDNA Viruses

Abstract

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Keywords

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