Flavobacterium soyangense sp. Nov., a psychrotolerant bacterium, isolated from an oligotrophic freshwater lake

A bacterial strain, designated IMCC26223^T, was isolated from an oligotrophic freshwater lake, Lake Soyang, Korea. Cells of strain IMCC26223^T were Gram-staining negative, strictly aerobic, non-motile and short-rod-shaped. Growth occurred at pH 6–8 (optimum, pH 7.0), at 4–25 °C (optimum, 15 °C) and with 0–0.5% (w/v) NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain IMCC26223^T was identified as a member of the genus Flavobacterium and most closely related to Flavobacterium fluvii H7^T (97.6 %), Flavobacterium segetis AT1048^T (97.5 %) and Flavobacterium weaverense AT1042^T (97.2 %). DNA–DNA relatedness between strain IMCC26223^T and F. fluvii H7^T was 41.5–51.7% in the reciprocal hybridization. Strain IMCC26223^T contained MK-6 as the major respiratory quinone. The major cellular fatty acids consisted of C_16:0, iso-C_15:0, anteiso-C_15:0 and summed feature 3 (C_16:1ω6c and/or C_16:1ω7c), and the polar lipids were phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. The DNA G+C content of strain IMCC26223^T was 34.5 mol%. On the basis of 16S rRNA gene phylogeny and phenotypic characterization, strain IMCC26223T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium soyangense sp. nov., is proposed. The type strain is IMCC26223^T (=KCTC 52245^T=JCM 31384^T).