Extraction of catechin compounds from green tea with a new green solvent

Heng Zhang; Baokun Tang; Kyungho Row

doi:10.1007/s40242-014-3339-0

Extraction of catechin compounds from green tea with a new green solvent

Heng Zhang, Baokun Tang, Kyungho Row

Inha University

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

An emerging green solvent called a deep eutectic solvent(DES) was applied to the extraction and determination of catechin(C), (+)epicatechin gallate(ECG) and (-)epigallocatechin gallate(EGCG) from Chinese green tea. After evaluating different combinations of them by extraction methods and DESs, a DES-based extraction method was established and optimized by a systematic investigation of the influencing factors. As a result, a total of 3.629, 35.25 or 114.2 mg/g catechin, (+)epicatechin gallate or (-)epigallocatechin gallate were extracted respectively under optimal conditions with extraction efficiencies of 82.7%, 92.3% and 97.0%, respectively. By comparing with other common used solvents for extracting catechin compounds, DESs were proved to be potential extraction solvents for bioactive ingredients.

Original language	English
Pages (from-to)	37-41
Number of pages	5
Journal	Chemical Research in Chinese Universities
Volume	30
Issue number	1
DOIs	https://doi.org/10.1007/s40242-014-3339-0
State	Published - Feb 2014

Bibliographical note

Funding Information:
——————————— *Corresponding author. E-mail: [email protected] Received August 9, 2013; accepted November 5, 2013. Supported by the Basic Science Research Program Through the National Research Foundation(NRF) of Korea Funded by the Ministry of Education, Science and Technology(No. 2011-0010673). © Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH solution(2000 μg/mL) and (–)epigallocatechin gallate(purity 95.0%) were purchased from Sigma(Saint Louis, USA). Ethylene glycol(purity>99.5%), glycerol(purity>99.0%), 1,2-butanediol(purity>98.0%), 1,3-butanediol(purity>99.0%), 1,4-butanediol(purity>99.0%), 2,3-butanediol(purity>97.0%), 1,6-hexanediol(purity>97.0%) and choline chloride(purity>98.0%) were obtained from Tokyo Chemical Industry Co., Ltd.(Tokyo, Japan). Methanol, ethanol, acetonitrile and hexane were supplied by DUKSAN Pure Chemical Co., Ltd.(Ansan, Korea). Distilled water was filtered via a vacuum pump(Division of Millipore, Waters, Billerica, USA) and a filter(HA-0.45, Division of Millipore, Waters, Bedford, USA). All the solvents used in the experiment were of HPLC or analytical grade. All the samples were filtered through a filter(MFS-25, 0.2 μm TF, WHATMAN, Piscataway, USA) before they were injected into the HPLC system.

Keywords

Catechin
Deep eutectic solvent
Extraction
High performance liquid chromatography

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10.1007/s40242-014-3339-0

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title = "Extraction of catechin compounds from green tea with a new green solvent",

abstract = "An emerging green solvent called a deep eutectic solvent(DES) was applied to the extraction and determination of catechin(C), (+)epicatechin gallate(ECG) and (-)epigallocatechin gallate(EGCG) from Chinese green tea. After evaluating different combinations of them by extraction methods and DESs, a DES-based extraction method was established and optimized by a systematic investigation of the influencing factors. As a result, a total of 3.629, 35.25 or 114.2 mg/g catechin, (+)epicatechin gallate or (-)epigallocatechin gallate were extracted respectively under optimal conditions with extraction efficiencies of 82.7%, 92.3% and 97.0%, respectively. By comparing with other common used solvents for extracting catechin compounds, DESs were proved to be potential extraction solvents for bioactive ingredients.",

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author = "Heng Zhang and Baokun Tang and Kyungho Row",

note = "Funding Information: ——————————— *Corresponding author. E-mail: [email protected] Received August 9, 2013; accepted November 5, 2013. Supported by the Basic Science Research Program Through the National Research Foundation(NRF) of Korea Funded by the Ministry of Education, Science and Technology(No. 2011-0010673). {\textcopyright} Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH solution(2000 μg/mL) and (–)epigallocatechin gallate(purity 95.0%) were purchased from Sigma(Saint Louis, USA). Ethylene glycol(purity>99.5%), glycerol(purity>99.0%), 1,2-butanediol(purity>98.0%), 1,3-butanediol(purity>99.0%), 1,4-butanediol(purity>99.0%), 2,3-butanediol(purity>97.0%), 1,6-hexanediol(purity>97.0%) and choline chloride(purity>98.0%) were obtained from Tokyo Chemical Industry Co., Ltd.(Tokyo, Japan). Methanol, ethanol, acetonitrile and hexane were supplied by DUKSAN Pure Chemical Co., Ltd.(Ansan, Korea). Distilled water was filtered via a vacuum pump(Division of Millipore, Waters, Billerica, USA) and a filter(HA-0.45, Division of Millipore, Waters, Bedford, USA). All the solvents used in the experiment were of HPLC or analytical grade. All the samples were filtered through a filter(MFS-25, 0.2 μm TF, WHATMAN, Piscataway, USA) before they were injected into the HPLC system.",

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doi = "10.1007/s40242-014-3339-0",

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AU - Tang, Baokun

AU - Row, Kyungho

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N2 - An emerging green solvent called a deep eutectic solvent(DES) was applied to the extraction and determination of catechin(C), (+)epicatechin gallate(ECG) and (-)epigallocatechin gallate(EGCG) from Chinese green tea. After evaluating different combinations of them by extraction methods and DESs, a DES-based extraction method was established and optimized by a systematic investigation of the influencing factors. As a result, a total of 3.629, 35.25 or 114.2 mg/g catechin, (+)epicatechin gallate or (-)epigallocatechin gallate were extracted respectively under optimal conditions with extraction efficiencies of 82.7%, 92.3% and 97.0%, respectively. By comparing with other common used solvents for extracting catechin compounds, DESs were proved to be potential extraction solvents for bioactive ingredients.

AB - An emerging green solvent called a deep eutectic solvent(DES) was applied to the extraction and determination of catechin(C), (+)epicatechin gallate(ECG) and (-)epigallocatechin gallate(EGCG) from Chinese green tea. After evaluating different combinations of them by extraction methods and DESs, a DES-based extraction method was established and optimized by a systematic investigation of the influencing factors. As a result, a total of 3.629, 35.25 or 114.2 mg/g catechin, (+)epicatechin gallate or (-)epigallocatechin gallate were extracted respectively under optimal conditions with extraction efficiencies of 82.7%, 92.3% and 97.0%, respectively. By comparing with other common used solvents for extracting catechin compounds, DESs were proved to be potential extraction solvents for bioactive ingredients.

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JO - Chemical Research in Chinese Universities

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Extraction of catechin compounds from green tea with a new green solvent

Abstract

Bibliographical note

Keywords

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