Extraction and HPLC analysis of tanshinone I, Tanshinone IIA and cryptotanshinone from salvia miltiorrhiza bunge

In the present work, simultaneous extraction and separation of tanshinone I, tanshinone IIA and cryptotanshinone has been developed and validated from Salvia miltiorrhiza bunge. The extracts of the tanshinones were separated on a C₁₈., column with the mobile phase composed of methanol-water (78:22, v/v, containing 0.5 % v acetic acid) at a flow-rate of 0.5 mL/min. The calibration was linear in a range of 0.1-500.0 μg/mL for these three compounds. The extracted amounts are 0.0091 mg/g for the tanshinone 1, 0.12 mg/g for the tanshinone IIA and 0.15 mg/g for the cryptotanshinone.