Evaluation of the wound-healing activity of rice cell extracts in vitro

Z. Hun Kim, Sun Mi Kim, Jin Ho Park, Chan Mi Park, Hong Yeol Choi, Hoomin Lee, Jae Kweon Park, Soonjo Kwon, Dong Il Kim, Kyu Ho Chang, Yong Soo Choi, Sang Min Lim

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

In the present study, we evaluated the in vitro wound-healing properties of two types of rice cell extracts (RCEs; prepared using ethanol and pressurized hot water extraction methods), using human dermal fibro-blasts and keratinocytes. The effects of the RCEs (at 25-100 μg/ml) on cytotoxicity and cell migration were assessed. Both RCEs were not cytotoxic to the two cell types, instead increasing their proliferation by up to 25% in a dose-dependent manner compared with the controls. Furthermore, both RCEs significantly enhanced the migratory ability of the two cell types (fibroblast, 230-450%; keratinocyte, 170-350%). Additionally, we examined the effect of the RCEs on type I collagen synthesis, which is important in the wound reconstruction process. The RCEs significantly increased collagen type I mRNA and protein levels to a degree comparable to that induced by Vitamin C. These results suggest the RCEs to be candidate materials for use in promoting wound healing, through their actions of increasing cell migration and accelerating wound re-epithelialization.

Original languageEnglish
Pages (from-to)285-292
Number of pages8
JournalMicrobiology and Biotechnology Letters
Volume44
Issue number3
DOIs
StatePublished - Sep 2016

Bibliographical note

Publisher Copyright:
© 2016, The Korean Society for Microbiology and Biotechnology.

Keywords

  • Collagen type I
  • Fibroblast
  • Keratinocyte
  • Rice cell extract
  • Wound healing

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