Emticicia fontis sp. Nov., isolated from a freshwater pond

A bacterial strain, designated IMCC1731^T, was isolated from an eutrophic freshwater pond and characterized using a polyphasic taxonomic approach. Cellular growth occurred at pH 6–9 (optimum, pH 8.0), at 10–30°C (optimum, 25°C) and with 0–1% (w/v) NaCl (optimum, 0%). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain IMCC1731^T belonged to the genus Emticicia in the family Cytophagaceae and was most closely related to Emticicia ginsengisoli Gsoil 085^T (98.1%) followed by Emticicia paludis HMF3850^T (95.0%), Emticicia oligotrophica DSM 17448^T (94.7%), Emticicia aquatica HMF2925^T (94.4%) and Emticicia sediminis JBR12^T (94.0%). The DNA–DNA relatedness between strain IMCC1731^T and E. ginsengisoli Gsoil 085^T was 47±4%. The DNA G+C content of strain IMCC1731^T was 37.7 mol%. The predominant cellular fatty acids were summed feature 3 (C_{16: 1}w6c and/or C_{16: 1}w7c) and iso-C_{15: 0}. Based on the physiological and chemotaxonomic characteristics, DNA–DNA relatedness and 16S rRNA gene phylogeny, stain IMCC1731^T is considered to represent a novel species of the genus Emticicia, for which the name Emticicia fontis sp. nov. is proposed. The type strain is IMCC1731^T (=KCTC 52248^T=JCM 31373^T).