Electron bifurcation reactions in dark fermentation: An overview for better understanding and improvement

Electron bifurcation (EB) is the most recently found mode of energy conservation, which involves both exergonic and endergonic electron transfer reactions to minimize energy loss. Several works have been devoted on EB reactions (EBRs) in anaerobic digestion but limited in dark fermentative hydrogen production (DF). Two main electron carriers in DF are ferredoxin (Fd) and reduced nicotinamide adenine dinucleotide (NADH), complicatedly involved in EB. Here, i) the importance of EB involvement in DF, ii) all EBRs possible to present in DF, as well as iii) the limitation of previous studies that tried incorporating any of EBRs in DF metabolic model, were highlighted. In addition, the concept of using metagenomic analysis for estimating the share of each EB reaction in the metabolic model, was proposed. This review is expected to initiate a new wave for studying EB, as a tool for explaining and predicting DF products.