Effects of various extracellular matrix proteins on the growth of HL-1 cardiomyocytes

Seongkyun Choi; Yoonmi Hong; Insu Lee; Dongeun Huh; Tae Joon Jeon; Sun Min Kim

doi:10.1159/000358755

Effects of various extracellular matrix proteins on the growth of HL-1 cardiomyocytes

Seongkyun Choi, Yoonmi Hong, Insu Lee, Dongeun Huh, Tae Joon Jeon, Sun Min Kim

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We present the physical and biochemical effects of extracellular matrixes (ECMs) on HL-1 cardiomyocytes. ECMs play major roles in cell growth, adhesion and the maintenance of native cell functions. We investigated the effects of 6 different cell culture systems: 5 different ECM-treated surfaces (fibronectin, laminin, collagen I, gelatin and a gelatin/fibronectin mixture) and 1 nontreated surface. Surface morphology was scanned and analyzed using atomic force microscopy in order to investigate the physical effects of ECMs. The attachment, growth, viability, proliferation and phenotype of the cells were analyzed using phase-contrast microscopy and immunocytochemistry to elucidate the biochemical effects of ECMs. Our study provides basic information for understanding cell-ECM interactions and should be utilized in future cardiac cell research and tissue engineering.

Original language	English
Pages (from-to)	349-356
Number of pages	8
Journal	Cells Tissues Organs
Volume	198
Issue number	5
DOIs	https://doi.org/10.1159/000358755
State	Published - 2014

Keywords

Cardiomyocytes
Cell culture systems
Extracellular matrix
HL-1 cells

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title = "Effects of various extracellular matrix proteins on the growth of HL-1 cardiomyocytes",

abstract = "We present the physical and biochemical effects of extracellular matrixes (ECMs) on HL-1 cardiomyocytes. ECMs play major roles in cell growth, adhesion and the maintenance of native cell functions. We investigated the effects of 6 different cell culture systems: 5 different ECM-treated surfaces (fibronectin, laminin, collagen I, gelatin and a gelatin/fibronectin mixture) and 1 nontreated surface. Surface morphology was scanned and analyzed using atomic force microscopy in order to investigate the physical effects of ECMs. The attachment, growth, viability, proliferation and phenotype of the cells were analyzed using phase-contrast microscopy and immunocytochemistry to elucidate the biochemical effects of ECMs. Our study provides basic information for understanding cell-ECM interactions and should be utilized in future cardiac cell research and tissue engineering.",

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AU - Choi, Seongkyun

AU - Hong, Yoonmi

AU - Lee, Insu

AU - Huh, Dongeun

AU - Jeon, Tae Joon

AU - Kim, Sun Min

PY - 2014

Y1 - 2014

N2 - We present the physical and biochemical effects of extracellular matrixes (ECMs) on HL-1 cardiomyocytes. ECMs play major roles in cell growth, adhesion and the maintenance of native cell functions. We investigated the effects of 6 different cell culture systems: 5 different ECM-treated surfaces (fibronectin, laminin, collagen I, gelatin and a gelatin/fibronectin mixture) and 1 nontreated surface. Surface morphology was scanned and analyzed using atomic force microscopy in order to investigate the physical effects of ECMs. The attachment, growth, viability, proliferation and phenotype of the cells were analyzed using phase-contrast microscopy and immunocytochemistry to elucidate the biochemical effects of ECMs. Our study provides basic information for understanding cell-ECM interactions and should be utilized in future cardiac cell research and tissue engineering.

AB - We present the physical and biochemical effects of extracellular matrixes (ECMs) on HL-1 cardiomyocytes. ECMs play major roles in cell growth, adhesion and the maintenance of native cell functions. We investigated the effects of 6 different cell culture systems: 5 different ECM-treated surfaces (fibronectin, laminin, collagen I, gelatin and a gelatin/fibronectin mixture) and 1 nontreated surface. Surface morphology was scanned and analyzed using atomic force microscopy in order to investigate the physical effects of ECMs. The attachment, growth, viability, proliferation and phenotype of the cells were analyzed using phase-contrast microscopy and immunocytochemistry to elucidate the biochemical effects of ECMs. Our study provides basic information for understanding cell-ECM interactions and should be utilized in future cardiac cell research and tissue engineering.

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KW - Cell culture systems

KW - Extracellular matrix

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Effects of various extracellular matrix proteins on the growth of HL-1 cardiomyocytes

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Keywords

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