Development of lateral flow assay based on size-controlled gold nanoparticles for detection of hepatitis B surface antigen

Dong Seok Kim; Yong Tae Kim; Seok Bok Hong; Jinwoon Kim; Nam Su Huh; Moon Keun Lee; Seok Jae Lee; Byeong Il Kim; In Soo Kim; Yun Suk Huh; Bong Gill Choi

doi:10.3390/s16122154

Development of lateral flow assay based on size-controlled gold nanoparticles for detection of hepatitis B surface antigen

Dong Seok Kim, Yong Tae Kim, Seok Bok Hong, Jinwoon Kim, Nam Su Huh, Moon Keun Lee, Seok Jae Lee, Byeong Il Kim, In Soo Kim, Yun Suk Huh, Bong Gill Choi

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342–137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 ± 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs.

Original language	English
Article number	2154
Journal	Sensors
Volume	16
Issue number	12
DOIs	https://doi.org/10.3390/s16122154
State	Published - 16 Dec 2016

Bibliographical note

Publisher Copyright:
© 2016 by the authors; licensee MDPI, Basel, Switzerland.

Keywords

Conjugation
Gold
Hepatitis B surface antigen
Lateral flow assay
Nanoparticle

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/s16122154

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title = "Development of lateral flow assay based on size-controlled gold nanoparticles for detection of hepatitis B surface antigen",

abstract = "In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342–137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 ± 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs.",

keywords = "Conjugation, Gold, Hepatitis B surface antigen, Lateral flow assay, Nanoparticle",

author = "Kim, {Dong Seok} and Kim, {Yong Tae} and Hong, {Seok Bok} and Jinwoon Kim and Huh, {Nam Su} and Lee, {Moon Keun} and Lee, {Seok Jae} and Kim, {Byeong Il} and Kim, {In Soo} and Huh, {Yun Suk} and Choi, {Bong Gill}",

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doi = "10.3390/s16122154",

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AU - Kim, Dong Seok

AU - Kim, Yong Tae

AU - Hong, Seok Bok

AU - Kim, Jinwoon

AU - Huh, Nam Su

AU - Lee, Moon Keun

AU - Lee, Seok Jae

AU - Kim, Byeong Il

AU - Kim, In Soo

AU - Huh, Yun Suk

AU - Choi, Bong Gill

PY - 2016/12/16

Y1 - 2016/12/16

N2 - In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342–137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 ± 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs.

AB - In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342–137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 ± 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs.

KW - Conjugation

KW - Gold

KW - Hepatitis B surface antigen

KW - Lateral flow assay

KW - Nanoparticle

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JO - Sensors

JF - Sensors

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ER -

Development of lateral flow assay based on size-controlled gold nanoparticles for detection of hepatitis B surface antigen

Abstract

Bibliographical note

Keywords

UN SDGs

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