Abstract
To determine the protein content of formula, gel electrophoresis was performed on the infant formula samples and the entire protein patterns were analyzed by nano-high performance liquid chromatography-electrospray tandem mass spectrometry (nano-HPLC/ESI/MS/MS). From the commercial infant formula profiled in this study, a total of 154 peptides, corresponding to 31 unique proteins were identified by nano-HPLC/ESI/MS/MS. Each of the identified peptides was reconfirmed by a strict integrated approach using tandem mass spectra. This protein profiling method using gel electrophoresis coupled with nano-HPLC/ESI/MS/MS and manual evaluation is a sensitive and accurate method for protein identification as well as a powerful tool for monitoring various types of food products.
| Original language | English |
|---|---|
| Pages (from-to) | 69-73 |
| Number of pages | 5 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 845 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Jan 2007 |
| Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by Grant no. R01-2006-000-10593-0 from the Basic Research Program of the Korea Science & Engineering Foundation.
Keywords
- Food analysis
- High-performance liquid chromatography
- Infant formula
- Tandem mass spectrometry