Desalting of urea from salt-rich protein sample facilitated by copper (II) ion in microfluidic decive

Kwang Suk Yang; Yun Suk Huh; Yeon Ki Hong; Won Hi Hong; Do Hyun Kim

Desalting of urea from salt-rich protein sample facilitated by copper (II) ion in microfluidic decive

Kwang Suk Yang, Yun Suk Huh, Yeon Ki Hong, Won Hi Hong, Do Hyun Kim

Research output: Contribution to conference › Paper › peer-review

Abstract

We present the simple and rapid microfluidic desalting systems, which are 3-phase desalting system using simple diffusion and (3+2)-phase desalting system based on the coupling of simple diffusion and affinity of urea. The facilitative desalting system is particularly useful for removal of urea and analysis of protein by mass spectroscopy because urea is removed by the mass transfer with the difference of concentration, molecular size and the affinity of urea to copper (II) ion resulting the complex. We also evaluated the activity and MALDI-TOF-MS spectrum of red fluorescent protein (RFP) for protein analysis.

Original language	English
Pages	888-890
Number of pages	3
State	Published - 2006
Externally published	Yes
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 5 Nov 2006 → 9 Nov 2006

Conference

Conference	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	5/11/06 → 9/11/06

Keywords

Desalting
Micro-dialysis
Protein pretreatment

Cite this

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title = "Desalting of urea from salt-rich protein sample facilitated by copper (II) ion in microfluidic decive",

abstract = "We present the simple and rapid microfluidic desalting systems, which are 3-phase desalting system using simple diffusion and (3+2)-phase desalting system based on the coupling of simple diffusion and affinity of urea. The facilitative desalting system is particularly useful for removal of urea and analysis of protein by mass spectroscopy because urea is removed by the mass transfer with the difference of concentration, molecular size and the affinity of urea to copper (II) ion resulting the complex. We also evaluated the activity and MALDI-TOF-MS spectrum of red fluorescent protein (RFP) for protein analysis.",

keywords = "Desalting, Micro-dialysis, Protein pretreatment",

author = "Yang, {Kwang Suk} and Huh, {Yun Suk} and Hong, {Yeon Ki} and Hong, {Won Hi} and Kim, {Do Hyun}",

year = "2006",

language = "English",

pages = "888--890",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

}

Desalting of urea from salt-rich protein sample facilitated by copper (II) ion in microfluidic decive. / Yang, Kwang Suk; Huh, Yun Suk; Hong, Yeon Ki et al.
2006. 888-890 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Desalting of urea from salt-rich protein sample facilitated by copper (II) ion in microfluidic decive

AU - Yang, Kwang Suk

AU - Huh, Yun Suk

AU - Hong, Yeon Ki

AU - Hong, Won Hi

AU - Kim, Do Hyun

PY - 2006

Y1 - 2006

N2 - We present the simple and rapid microfluidic desalting systems, which are 3-phase desalting system using simple diffusion and (3+2)-phase desalting system based on the coupling of simple diffusion and affinity of urea. The facilitative desalting system is particularly useful for removal of urea and analysis of protein by mass spectroscopy because urea is removed by the mass transfer with the difference of concentration, molecular size and the affinity of urea to copper (II) ion resulting the complex. We also evaluated the activity and MALDI-TOF-MS spectrum of red fluorescent protein (RFP) for protein analysis.

AB - We present the simple and rapid microfluidic desalting systems, which are 3-phase desalting system using simple diffusion and (3+2)-phase desalting system based on the coupling of simple diffusion and affinity of urea. The facilitative desalting system is particularly useful for removal of urea and analysis of protein by mass spectroscopy because urea is removed by the mass transfer with the difference of concentration, molecular size and the affinity of urea to copper (II) ion resulting the complex. We also evaluated the activity and MALDI-TOF-MS spectrum of red fluorescent protein (RFP) for protein analysis.

KW - Desalting

KW - Micro-dialysis

KW - Protein pretreatment

UR - http://www.scopus.com/inward/record.url?scp=84902474826&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902474826

SP - 888

EP - 890

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Desalting of urea from salt-rich protein sample facilitated by copper (II) ion in microfluidic decive

Abstract

Conference

Keywords

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