Colorimetric detection of ssDNA in a solution

Sangchul Rho; Sung Joong Kim; Sang Cheon Lee; Jeong Ho Chang; Hee Gyoo Kang; Jinsub Choi

doi:10.1016/j.cap.2008.03.025

Colorimetric detection of ssDNA in a solution

Sangchul Rho, Sung Joong Kim, Sang Cheon Lee, Jeong Ho Chang, Hee Gyoo Kang, Jinsub Choi

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

A solution containing ssDNA was detected by using colloidal gold nanoparticles upon a color change of solution. First of all, we prepared colloidal gold nanoparticles with a size of less than 20 nm by means of citrate reduction of HAuCl₄. Since colloidal gold nanoparticles modified with citrate anions have a negative charge, they are very well dispersed in the solution due to the negative charge repulsion, showing red color. If the electrostatic repulsion is screened by additives such as NaCl, the nanoparticles start to aggregate, leading to a color alteration. We found that the color alteration is retarded when the solution contains ssDNA, which plays a role in preventing the nanoparticles from aggregation. This allows the determination of whether or not ssDNA is present in a solution. However, the color alteration is not retarded when the solution contains dsDNA.

Original language	English
Pages (from-to)	534-537
Number of pages	4
Journal	Current Applied Physics
Volume	9
Issue number	2
DOIs	https://doi.org/10.1016/j.cap.2008.03.025
State	Published - Mar 2009
Externally published	Yes

Bibliographical note

Funding Information:
This work was in part supported by the Bio-Medtech Regional Innovation Center at Eulji University, under the Regional Innovation Center Program of Ministry of Commerce, Industry and Energy, and was in part supported by the Korea Science and Engineering Foundation (KOSEF) Grant funded by the Korean government (MOST) (R01-2007-000-20290-0).

Keywords

Color
DNA sensors
Gold nanoparticles

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10.1016/j.cap.2008.03.025

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title = "Colorimetric detection of ssDNA in a solution",

abstract = "A solution containing ssDNA was detected by using colloidal gold nanoparticles upon a color change of solution. First of all, we prepared colloidal gold nanoparticles with a size of less than 20 nm by means of citrate reduction of HAuCl4. Since colloidal gold nanoparticles modified with citrate anions have a negative charge, they are very well dispersed in the solution due to the negative charge repulsion, showing red color. If the electrostatic repulsion is screened by additives such as NaCl, the nanoparticles start to aggregate, leading to a color alteration. We found that the color alteration is retarded when the solution contains ssDNA, which plays a role in preventing the nanoparticles from aggregation. This allows the determination of whether or not ssDNA is present in a solution. However, the color alteration is not retarded when the solution contains dsDNA.",

keywords = "Color, DNA sensors, Gold nanoparticles",

author = "Sangchul Rho and Kim, {Sung Joong} and Lee, {Sang Cheon} and Chang, {Jeong Ho} and Kang, {Hee Gyoo} and Jinsub Choi",

note = "Funding Information: This work was in part supported by the Bio-Medtech Regional Innovation Center at Eulji University, under the Regional Innovation Center Program of Ministry of Commerce, Industry and Energy, and was in part supported by the Korea Science and Engineering Foundation (KOSEF) Grant funded by the Korean government (MOST) (R01-2007-000-20290-0).",

year = "2009",

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doi = "10.1016/j.cap.2008.03.025",

language = "English",

volume = "9",

pages = "534--537",

journal = "Current Applied Physics",

issn = "1567-1739",

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AU - Rho, Sangchul

AU - Kim, Sung Joong

AU - Lee, Sang Cheon

AU - Chang, Jeong Ho

AU - Kang, Hee Gyoo

AU - Choi, Jinsub

N1 - Funding Information: This work was in part supported by the Bio-Medtech Regional Innovation Center at Eulji University, under the Regional Innovation Center Program of Ministry of Commerce, Industry and Energy, and was in part supported by the Korea Science and Engineering Foundation (KOSEF) Grant funded by the Korean government (MOST) (R01-2007-000-20290-0).

PY - 2009/3

Y1 - 2009/3

N2 - A solution containing ssDNA was detected by using colloidal gold nanoparticles upon a color change of solution. First of all, we prepared colloidal gold nanoparticles with a size of less than 20 nm by means of citrate reduction of HAuCl4. Since colloidal gold nanoparticles modified with citrate anions have a negative charge, they are very well dispersed in the solution due to the negative charge repulsion, showing red color. If the electrostatic repulsion is screened by additives such as NaCl, the nanoparticles start to aggregate, leading to a color alteration. We found that the color alteration is retarded when the solution contains ssDNA, which plays a role in preventing the nanoparticles from aggregation. This allows the determination of whether or not ssDNA is present in a solution. However, the color alteration is not retarded when the solution contains dsDNA.

AB - A solution containing ssDNA was detected by using colloidal gold nanoparticles upon a color change of solution. First of all, we prepared colloidal gold nanoparticles with a size of less than 20 nm by means of citrate reduction of HAuCl4. Since colloidal gold nanoparticles modified with citrate anions have a negative charge, they are very well dispersed in the solution due to the negative charge repulsion, showing red color. If the electrostatic repulsion is screened by additives such as NaCl, the nanoparticles start to aggregate, leading to a color alteration. We found that the color alteration is retarded when the solution contains ssDNA, which plays a role in preventing the nanoparticles from aggregation. This allows the determination of whether or not ssDNA is present in a solution. However, the color alteration is not retarded when the solution contains dsDNA.

KW - Color

KW - DNA sensors

KW - Gold nanoparticles

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DO - 10.1016/j.cap.2008.03.025

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SN - 1567-1739

VL - 9

SP - 534

EP - 537

JO - Current Applied Physics

JF - Current Applied Physics

IS - 2

ER -

Colorimetric detection of ssDNA in a solution

Abstract

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Keywords

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