Chromatographic analysis of ceramide III of Saccharomyces cerevisiae

Mi Soon Yeo; Duk Hui Kang; Se Kyung Kim; Hyun Shik Yun; Yoon Mo Koo; Youn Woo Lee; Kyung Ho Row

doi:10.1007/BF02706973

Chromatographic analysis of ceramide III of Saccharomyces cerevisiae

Mi Soon Yeo, Duk Hui Kang, Se Kyung Kim, Hyun Shik Yun, Yoon Mo Koo, Youn Woo Lee, Kyung Ho Row

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Ceramide was prepared by the cultivation of Saccharomyces cerevisiae from cell extracts by solvent extraction and analyzed by NP-HPLC using a UV detector. The mobile phase was composed of hexane, methanol, and IPA. From the experimental conditions, the composition of mobile phase was 72/5/23 (hexane/IPA/methanol, vol%). Quantitative analysis of ceramide was performed. Based on the analytical conditions, the effect of cultivation temperature for the production of ceramide was investigated and the optimum cultivation temperature was found to be 35 °C.

Original language	English
Pages (from-to)	815-817
Number of pages	3
Journal	Korean Journal of Chemical Engineering
Volume	19
Issue number	5
DOIs	https://doi.org/10.1007/BF02706973
State	Published - Sep 2002

Bibliographical note

Funding Information:
The authors were gratefully appreciated for the financial support by KRF (2001-042-E00072)

Keywords

Ceramide
Preparative HPLC
Saccharomyces cerevisiae

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10.1007/BF02706973

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@article{29a3b818a3034123a712e10113634e19,

title = "Chromatographic analysis of ceramide III of Saccharomyces cerevisiae",

abstract = "Ceramide was prepared by the cultivation of Saccharomyces cerevisiae from cell extracts by solvent extraction and analyzed by NP-HPLC using a UV detector. The mobile phase was composed of hexane, methanol, and IPA. From the experimental conditions, the composition of mobile phase was 72/5/23 (hexane/IPA/methanol, vol%). Quantitative analysis of ceramide was performed. Based on the analytical conditions, the effect of cultivation temperature for the production of ceramide was investigated and the optimum cultivation temperature was found to be 35 °C.",

keywords = "Ceramide, Preparative HPLC, Saccharomyces cerevisiae",

author = "Yeo, {Mi Soon} and Kang, {Duk Hui} and Kim, {Se Kyung} and Yun, {Hyun Shik} and Koo, {Yoon Mo} and Lee, {Youn Woo} and Row, {Kyung Ho}",

note = "Funding Information: The authors were gratefully appreciated for the financial support by KRF (2001-042-E00072)",

year = "2002",

month = sep,

doi = "10.1007/BF02706973",

language = "English",

volume = "19",

pages = "815--817",

journal = "Korean Journal of Chemical Engineering",

issn = "0256-1115",

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TY - JOUR

T1 - Chromatographic analysis of ceramide III of Saccharomyces cerevisiae

AU - Yeo, Mi Soon

AU - Kang, Duk Hui

AU - Kim, Se Kyung

AU - Yun, Hyun Shik

AU - Koo, Yoon Mo

AU - Lee, Youn Woo

AU - Row, Kyung Ho

N1 - Funding Information: The authors were gratefully appreciated for the financial support by KRF (2001-042-E00072)

PY - 2002/9

Y1 - 2002/9

N2 - Ceramide was prepared by the cultivation of Saccharomyces cerevisiae from cell extracts by solvent extraction and analyzed by NP-HPLC using a UV detector. The mobile phase was composed of hexane, methanol, and IPA. From the experimental conditions, the composition of mobile phase was 72/5/23 (hexane/IPA/methanol, vol%). Quantitative analysis of ceramide was performed. Based on the analytical conditions, the effect of cultivation temperature for the production of ceramide was investigated and the optimum cultivation temperature was found to be 35 °C.

AB - Ceramide was prepared by the cultivation of Saccharomyces cerevisiae from cell extracts by solvent extraction and analyzed by NP-HPLC using a UV detector. The mobile phase was composed of hexane, methanol, and IPA. From the experimental conditions, the composition of mobile phase was 72/5/23 (hexane/IPA/methanol, vol%). Quantitative analysis of ceramide was performed. Based on the analytical conditions, the effect of cultivation temperature for the production of ceramide was investigated and the optimum cultivation temperature was found to be 35 °C.

KW - Ceramide

KW - Preparative HPLC

KW - Saccharomyces cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=23044533436&partnerID=8YFLogxK

U2 - 10.1007/BF02706973

DO - 10.1007/BF02706973

M3 - Article

AN - SCOPUS:23044533436

SN - 0256-1115

VL - 19

SP - 815

EP - 817

JO - Korean Journal of Chemical Engineering

JF - Korean Journal of Chemical Engineering

IS - 5

ER -

Chromatographic analysis of ceramide III of Saccharomyces cerevisiae

Abstract

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Keywords

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