Abstract
A water-soluble polysaccharide was isolated and purified from the culture filtrate of the photosynthetic green microalgae Haematococcus lacustris by 75% ethanol precipitation and Sepharose CL-6B column chromatography. The molecular mass of the purified polysaccharide (named HCP) was estimated to be approximately 135 kDa by sizeexclusion HPLC and its monosaccharide composition was galactose, glucose and mannose at a relative molar ratio of 2.0, 1.0, and 4.1, respectively, suggesting that HCP is a galactomannan. Fourier-transform infrared and elemental analysis revealed that the purified HCP contains sulfate esters by 1.08% (in mass) and no detectable level of protein. The HCP significantly stimulated murine macrophage RAW264.7 cells to secrete the pro-inflammatory cytokine, TNF-α, in a dose-dependent manner and also enhanced the expression of COX-2 and iNOS genes at a concentration of lower than 10 μg/mL HCP. These results indicated that the sulfated HCP of H. lacustris has potent early innate immune stimulating activities.
| Original language | English |
|---|---|
| Pages (from-to) | 1090-1098 |
| Number of pages | 9 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume | 16 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 2011 |
Bibliographical note
Funding Information:This work was supported by a grant from the Gyeonggi-do GRRC program and the Research Fund, 2010 of The Catholic University of Korea, Korea, and partly by the Research Fund of The Gachon University of Medicine and Science, Korea, and a Manpower Development Program for Marine Energy by the Ministry of Land, Transport and Maritime Affairs (MLTM) for which the authors are thankful. The authors would like to thank Dr. Mawadda Alnaeeli, NIDDK, National Institutes of Health, Bethesda, MD, for her kindly reviewing the manuscript.
Keywords
- Haematococcus lacustris
- Immuno-stimulating activity
- Microalgae
- Polysaccharide