TY - JOUR
T1 - Carotenogenesis in Haematococcus lacustris
T2 - Role of protein tyrosine phosphatases
AU - Park, Jae Kweon
AU - Tran, Phuong Ngoc
AU - Kim, Jeong Dong
AU - Hong, Seong Joo
AU - Lee, Choul Gyun
PY - 2009
Y1 - 2009
N2 - In the present study, we examined the inhibitory effects of protein tyrosine phosphatase (PTPase) inhibitors, including sodium orthovanadate (SOV), ammonium molybdate (AM), and iodoacetamide (IA), on cell growth, accumulation of astaxanthin, and PTPase activity in the photosynthetic algae Haematococcus lacustris. PTPase activity was assayed spectrophotometrically and was found to be inhibited 60% to 90% after treatment with the inhibitors. SOV markedly abolished PTPase activity, significantly activating the accumulation of astaxanthin. These data suggest that the accumulation of astaxanthin in H. lacustris results from the concerted actions of several PTPases.
AB - In the present study, we examined the inhibitory effects of protein tyrosine phosphatase (PTPase) inhibitors, including sodium orthovanadate (SOV), ammonium molybdate (AM), and iodoacetamide (IA), on cell growth, accumulation of astaxanthin, and PTPase activity in the photosynthetic algae Haematococcus lacustris. PTPase activity was assayed spectrophotometrically and was found to be inhibited 60% to 90% after treatment with the inhibitors. SOV markedly abolished PTPase activity, significantly activating the accumulation of astaxanthin. These data suggest that the accumulation of astaxanthin in H. lacustris results from the concerted actions of several PTPases.
KW - Astaxanthin
KW - Haematococcus lacustris
KW - PTPase inhibitor
KW - Protein tyrosine phosphatase (PTPase)
KW - Sodium orthovanadate (SOV)
UR - http://www.scopus.com/inward/record.url?scp=70849133981&partnerID=8YFLogxK
U2 - 10.4014/jmb.0812.700
DO - 10.4014/jmb.0812.700
M3 - Article
C2 - 19809248
AN - SCOPUS:70849133981
SN - 1017-7825
VL - 19
SP - 918
EP - 921
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
IS - 9
ER -