TY - GEN
T1 - Carbon nanotube-collagen scaffolds for mesenchymal stem cells' differentiation
T2 - Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
AU - Baktur, R.
AU - Kwon, S.
AU - Sharma, R.
AU - Sharma, A.
PY - 2010
Y1 - 2010
N2 - Carbon nanotubes (CNTs) are of interest for biological and medical applications. The applications of carbon nanomaterials have tremendous potentials for them as substrates of cell cultures, drug delivery systems, and medical implantable materials. However, little is known about the impact of CNTs on cellular processes such as adhesion, proliferation, and differentiation. We hypothesized that augmenting the properties of naturally derived polymers (collagen, in this study) through incorporation of multi-walled CNTs (MWCNTs) might enhance in vitro osteogenic and osteoblastic differentiation of mesenchymal stem cells (MSCs). We used reconstituted Type I collagen and several different types of MWCNTs (MWCNT-COOH, MWCNT-OH, MWCNT-long and MWCNT-short). MSCs were incorporated at the time of scaffold preparation, creating a living-tissue analog consisting of cells embedded in a MWCNT-collagen scaffolds. MSCs were isolated from rat femur. When osteoprogenitor cells (or pre-osteoblast cells) reach confluence, the cell culture media was replaced with differentiation media containing 10 mM of β-glycerophosphate, 50 μg/ml of ascorbic acid, and 10 nM of dexamethasone. The cell proliferation, differentiation, mineralization and inflammatory response of MSCs were evaluated in the MWCNT-Collagen scaffolds. Alkaline phosphatase (AP) and mineralization of extracellular matrix (ECM) were monitored as osteoblastic and osteogenic differentiation markers. AP activity was increased as cells were differentiated. AP enzyme activity was significantly increased 12 days after replacement with differentiating media in the presence of MWCNT-Collagen scaffolds. The collagen interaction with MSCs enhanced AP activity, and MWCNTs induced further increase in AP activity. MWCNT-collagen scaffolds-induced AP activities were dose independent. The MWCNT-Collagen scaffolds showed different level of energy, but favorable energy conformations suitable as polymeric cages and scaffolds. This study showed the possibility of enhancement in MSC differentiation in the MWCNT-Collagen scaffolds.
AB - Carbon nanotubes (CNTs) are of interest for biological and medical applications. The applications of carbon nanomaterials have tremendous potentials for them as substrates of cell cultures, drug delivery systems, and medical implantable materials. However, little is known about the impact of CNTs on cellular processes such as adhesion, proliferation, and differentiation. We hypothesized that augmenting the properties of naturally derived polymers (collagen, in this study) through incorporation of multi-walled CNTs (MWCNTs) might enhance in vitro osteogenic and osteoblastic differentiation of mesenchymal stem cells (MSCs). We used reconstituted Type I collagen and several different types of MWCNTs (MWCNT-COOH, MWCNT-OH, MWCNT-long and MWCNT-short). MSCs were incorporated at the time of scaffold preparation, creating a living-tissue analog consisting of cells embedded in a MWCNT-collagen scaffolds. MSCs were isolated from rat femur. When osteoprogenitor cells (or pre-osteoblast cells) reach confluence, the cell culture media was replaced with differentiation media containing 10 mM of β-glycerophosphate, 50 μg/ml of ascorbic acid, and 10 nM of dexamethasone. The cell proliferation, differentiation, mineralization and inflammatory response of MSCs were evaluated in the MWCNT-Collagen scaffolds. Alkaline phosphatase (AP) and mineralization of extracellular matrix (ECM) were monitored as osteoblastic and osteogenic differentiation markers. AP activity was increased as cells were differentiated. AP enzyme activity was significantly increased 12 days after replacement with differentiating media in the presence of MWCNT-Collagen scaffolds. The collagen interaction with MSCs enhanced AP activity, and MWCNTs induced further increase in AP activity. MWCNT-collagen scaffolds-induced AP activities were dose independent. The MWCNT-Collagen scaffolds showed different level of energy, but favorable energy conformations suitable as polymeric cages and scaffolds. This study showed the possibility of enhancement in MSC differentiation in the MWCNT-Collagen scaffolds.
KW - Alkaline phosphatase
KW - Carbon nanotubes
KW - Collagen
KW - Energy content
KW - Mesenchymal stem cells
KW - Nano-scale scaffold
UR - http://www.scopus.com/inward/record.url?scp=78049422387&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:78049422387
SN - 9781439834152
T3 - Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
SP - 230
EP - 233
BT - Nanotechnology 2010
Y2 - 21 June 2010 through 24 June 2010
ER -