Bacterial community analyses by pyrosequencing in dark fermentative H ₂-producing reactor using organic wastes as a feedstock

TY - JOUR

T1 - Bacterial community analyses by pyrosequencing in dark fermentative H 2-producing reactor using organic wastes as a feedstock

AU - Im, Wan Taek

AU - Kim, Dong Hoon

AU - Kim, Kyung Ho

AU - Kim, Mi Sun

PY - 2012/5

Y1 - 2012/5

N2 - Hydrogen(H 2)-producing bacterial community structures of the dark fermentation system in a batch reactor were investigated during 48 h by analyzing 16S rRNA gene sequences obtained from pyrosequencing. Organic wastes composed of food waste and sewage sludge were used as a feedstock. After heat treatment (90°C for 20 min) of the feedstock, H 2 was naturally evolved under anaerobic mesophilic conditions, showing a H 2 yield of 2.26 mol H 2/mol hexose added. The bacterial community structure of the initial inoculum (microbial community at the starting point (0 h)) combined with heat treated food waste and sewage sludge was mainly comprised of Proteobacteria and Bacteroidetes. After 6 h operation, the sequences that belong to other groups except Firmicutes decreased dramatically and were not observed at all in the latter samples. Clostridium spp., which were negligible in the inoculum, took over the main bacterial community by taking charge of H 2 production. Among the phylum Firmicutes, the sequences closely related with Clostridium sordellii ATCC 9714 T, Clostridium perfringens ATCC 13124 T, and Clostridium butyricum ATCC 19398 T became predominant in the time series within 48 h. Overall, the results showed how fast the Clostridium spp. overwhelmed the bacterial community in dark fermentative H 2 production conditions, where they were at a negligible amount at the start.

AB - Hydrogen(H 2)-producing bacterial community structures of the dark fermentation system in a batch reactor were investigated during 48 h by analyzing 16S rRNA gene sequences obtained from pyrosequencing. Organic wastes composed of food waste and sewage sludge were used as a feedstock. After heat treatment (90°C for 20 min) of the feedstock, H 2 was naturally evolved under anaerobic mesophilic conditions, showing a H 2 yield of 2.26 mol H 2/mol hexose added. The bacterial community structure of the initial inoculum (microbial community at the starting point (0 h)) combined with heat treated food waste and sewage sludge was mainly comprised of Proteobacteria and Bacteroidetes. After 6 h operation, the sequences that belong to other groups except Firmicutes decreased dramatically and were not observed at all in the latter samples. Clostridium spp., which were negligible in the inoculum, took over the main bacterial community by taking charge of H 2 production. Among the phylum Firmicutes, the sequences closely related with Clostridium sordellii ATCC 9714 T, Clostridium perfringens ATCC 13124 T, and Clostridium butyricum ATCC 19398 T became predominant in the time series within 48 h. Overall, the results showed how fast the Clostridium spp. overwhelmed the bacterial community in dark fermentative H 2 production conditions, where they were at a negligible amount at the start.

KW - 16S rRNA gene

KW - Bacterial community

KW - Food waste

KW - Hydrogen fermentation

KW - Pyrosequencing

KW - Sewage sludge

UR - http://www.scopus.com/inward/record.url?scp=84860359379&partnerID=8YFLogxK

U2 - 10.1016/j.ijhydene.2012.02.167

DO - 10.1016/j.ijhydene.2012.02.167

M3 - Article

AN - SCOPUS:84860359379

SN - 0360-3199

VL - 37

SP - 8330

EP - 8337

JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

IS - 10

ER -