Abstract
Here we present an optofluidic surface enhanced Raman spectroscopy (SERS) device for on-chip detection of vasopressin using an aptamer based binding assay. To create the SERS-active substrate, densely packed, 200 nm diameter, metal nanotube arrays were fabricated using an anodized alumina nanoporous membrane as a template for shadow evaporation. We explore the use of both single layer Au structures and multilayer Au/Ag/Au structures and also demonstrate a facile technique for integrating the membranes with all polydimethylsiloxane (PDMS) microfluidic devices. Using the integrated device, we demonstrate a linear response in the main detection peak intensity to solution phase concentration and a limit of detection on the order of 5.2 μU/mL. This low limit of detection is obtained with device containing the multilayer SERS substrate which we show exhibits a stronger Raman enhancement while maintaining biocompatibility and ease or surface reactivity with the capture probe.
| Original language | English |
|---|---|
| Pages (from-to) | 1240-1243 |
| Number of pages | 4 |
| Journal | Biosensors and Bioelectronics |
| Volume | 25 |
| Issue number | 5 |
| DOIs | |
| State | Published - 15 Jan 2010 |
| Externally published | Yes |
Bibliographical note
Funding Information:Support for this work was provided by the National Institutes of Health – National Institute of Biomedical Imaging and Bioengineering (NIH-NIBIB) under grant number R21EB007031 . The authors also appreciate access and use of the Cornell Nanoscale Science and Technology Facility (a member of the National Nanofabrication Infrastructure Network), which is supported by the National Science Foundation under grant ECS-9731293 .
Keywords
- Aptamer
- Nanotube array
- Optofluidic device
- SERS-active substrate
- Vasopressin