Abstract
Chitosan derivatives have been studied widely, but poor solubility in water restricts their applications. In this study, four types of amine-based chitosan derivatives were prepared and modified further with beta-cyclodextrin. The sequential microextraction of catechins ((+)-catechin and (−)-epigallocatechin gallate) from green tea powder by an optimized solid-phase extraction method using these four derivatives was investigated. The optimal conditions for the extraction of catechins were 60°C for a 40 min extraction period. The purity and amount of each catechin were determined by high-performance liquid chromatography. The different amines strengthened the extraction capacity of chitosan. Among the four types of amines, ethylene diamine grafted chitosan beta-cyclodextrin had the highest extraction capacity to catechins. Therefore, this material was used in the extraction assay, and the standard curves of (+)-catechin and (−)-epigallocatechin gallate were linear over the concentration range, 0.25–500 µg/mL, after assaying five data points in duplicate. Solid-phase extraction with the amino-based chitosan beta-cyclodextrin system is a new application of chitosan, which has potential applications in the extraction of bioactive compounds from plant materials or the removal of different impurities from specific extracts.
Original language | English |
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Pages (from-to) | 2660-2667 |
Number of pages | 8 |
Journal | Journal of Separation Science |
Volume | 42 |
Issue number | 16 |
DOIs | |
State | Published - 2019 |
Bibliographical note
Publisher Copyright:© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Keywords
- catechins
- chitosan
- cyclodextrins
- green tea
- solid-phase microextraction