Anti-cancer effects of a novel compound HS-113 on cell growth, apoptosis, and angiogenesis in human hepatocellular carcinoma cells

Myung Joo Choi; Kyung Hee Jung; Donghee Kim; Hyunseung Lee; Hong Mei Zheng; Byung Hee Park; Sang Won Hong; Mi Hyun Kim; Sungwoo Hong; Soon Sun Hong

doi:10.1016/j.canlet.2011.03.005

Anti-cancer effects of a novel compound HS-113 on cell growth, apoptosis, and angiogenesis in human hepatocellular carcinoma cells

Myung Joo Choi, Kyung Hee Jung, Donghee Kim, Hyunseung Lee, Hong Mei Zheng, Byung Hee Park, Sang Won Hong, Mi Hyun Kim, Sungwoo Hong, Soon Sun Hong

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Hepatocellular carcinoma (HCC) is one of the most common malignancies, yet there have been no significant advances in effective therapeutics. In this study, HS-113 was synthesized as a novel compound, N-(5-(2-bromobenzyl) thiazole-2-yl) benzofuran-2-carboxamide and its cytotoxic activity and anti-cancer effect were examined in human HCC cells. HS-113 strongly suppressed growth of HCC cells in a dose-dependent manner, induced apoptosis by increasing the proportion of sub-G1 apoptotic cells, and caused cell cycle arrest at G0/G1 phase. Also, HS-113 increased the expression of p27 and decreased that of cyclin D1 associated with cell cycle arrest. Apoptosis by HS-113 was confirmed by DAPI and TUNEL staining, and the increases of the cleaved PARP and caspase-3 were observed. Furthermore, HS-113 decreased protein expression of HIF-1α and secretion of VEGF, and inhibited the tube formation of HUVECs. These results showed that HS-113 not only inhibited cell growth and angiogenesis, but also induced apoptosis of human HCC cells. We suggest that HS-113 may be a potential candidate for caner therapy against HCC.

Original language	English
Pages (from-to)	190-196
Number of pages	7
Journal	Cancer Letters
Volume	306
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2011.03.005
State	Published - 28 Jul 2011
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Korean Health Technology R&D Project (A101185) and the National R&D Program for Cancer Control (1020250), Ministry of Health & Welfare, and National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF 2010-0011895, 0015340, and 0022179).

Keywords

Angiogenesis
Apoptosis
HCC
HS-113

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2011.03.005

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title = "Anti-cancer effects of a novel compound HS-113 on cell growth, apoptosis, and angiogenesis in human hepatocellular carcinoma cells",

abstract = "Hepatocellular carcinoma (HCC) is one of the most common malignancies, yet there have been no significant advances in effective therapeutics. In this study, HS-113 was synthesized as a novel compound, N-(5-(2-bromobenzyl) thiazole-2-yl) benzofuran-2-carboxamide and its cytotoxic activity and anti-cancer effect were examined in human HCC cells. HS-113 strongly suppressed growth of HCC cells in a dose-dependent manner, induced apoptosis by increasing the proportion of sub-G1 apoptotic cells, and caused cell cycle arrest at G0/G1 phase. Also, HS-113 increased the expression of p27 and decreased that of cyclin D1 associated with cell cycle arrest. Apoptosis by HS-113 was confirmed by DAPI and TUNEL staining, and the increases of the cleaved PARP and caspase-3 were observed. Furthermore, HS-113 decreased protein expression of HIF-1α and secretion of VEGF, and inhibited the tube formation of HUVECs. These results showed that HS-113 not only inhibited cell growth and angiogenesis, but also induced apoptosis of human HCC cells. We suggest that HS-113 may be a potential candidate for caner therapy against HCC.",

keywords = "Angiogenesis, Apoptosis, HCC, HS-113",

author = "Choi, {Myung Joo} and Jung, {Kyung Hee} and Donghee Kim and Hyunseung Lee and Zheng, {Hong Mei} and Park, {Byung Hee} and Hong, {Sang Won} and Kim, {Mi Hyun} and Sungwoo Hong and Hong, {Soon Sun}",

note = "Funding Information: This work was supported by the Korean Health Technology R&D Project (A101185) and the National R&D Program for Cancer Control (1020250), Ministry of Health & Welfare, and National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF 2010-0011895, 0015340, and 0022179).",

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AU - Choi, Myung Joo

AU - Jung, Kyung Hee

AU - Kim, Donghee

AU - Lee, Hyunseung

AU - Zheng, Hong Mei

AU - Park, Byung Hee

AU - Hong, Sang Won

AU - Kim, Mi Hyun

AU - Hong, Sungwoo

AU - Hong, Soon Sun

N1 - Funding Information: This work was supported by the Korean Health Technology R&D Project (A101185) and the National R&D Program for Cancer Control (1020250), Ministry of Health & Welfare, and National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF 2010-0011895, 0015340, and 0022179).

PY - 2011/7/28

Y1 - 2011/7/28

N2 - Hepatocellular carcinoma (HCC) is one of the most common malignancies, yet there have been no significant advances in effective therapeutics. In this study, HS-113 was synthesized as a novel compound, N-(5-(2-bromobenzyl) thiazole-2-yl) benzofuran-2-carboxamide and its cytotoxic activity and anti-cancer effect were examined in human HCC cells. HS-113 strongly suppressed growth of HCC cells in a dose-dependent manner, induced apoptosis by increasing the proportion of sub-G1 apoptotic cells, and caused cell cycle arrest at G0/G1 phase. Also, HS-113 increased the expression of p27 and decreased that of cyclin D1 associated with cell cycle arrest. Apoptosis by HS-113 was confirmed by DAPI and TUNEL staining, and the increases of the cleaved PARP and caspase-3 were observed. Furthermore, HS-113 decreased protein expression of HIF-1α and secretion of VEGF, and inhibited the tube formation of HUVECs. These results showed that HS-113 not only inhibited cell growth and angiogenesis, but also induced apoptosis of human HCC cells. We suggest that HS-113 may be a potential candidate for caner therapy against HCC.

AB - Hepatocellular carcinoma (HCC) is one of the most common malignancies, yet there have been no significant advances in effective therapeutics. In this study, HS-113 was synthesized as a novel compound, N-(5-(2-bromobenzyl) thiazole-2-yl) benzofuran-2-carboxamide and its cytotoxic activity and anti-cancer effect were examined in human HCC cells. HS-113 strongly suppressed growth of HCC cells in a dose-dependent manner, induced apoptosis by increasing the proportion of sub-G1 apoptotic cells, and caused cell cycle arrest at G0/G1 phase. Also, HS-113 increased the expression of p27 and decreased that of cyclin D1 associated with cell cycle arrest. Apoptosis by HS-113 was confirmed by DAPI and TUNEL staining, and the increases of the cleaved PARP and caspase-3 were observed. Furthermore, HS-113 decreased protein expression of HIF-1α and secretion of VEGF, and inhibited the tube formation of HUVECs. These results showed that HS-113 not only inhibited cell growth and angiogenesis, but also induced apoptosis of human HCC cells. We suggest that HS-113 may be a potential candidate for caner therapy against HCC.

KW - Angiogenesis

KW - Apoptosis

KW - HCC

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Anti-cancer effects of a novel compound HS-113 on cell growth, apoptosis, and angiogenesis in human hepatocellular carcinoma cells

Abstract

Bibliographical note

Keywords

UN SDGs

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